The improper disposal of textile dyes like Rhodamine B into nearby water bodies and land areas affects living organisms nearby. Here, we explore the effects of rhodamine absorption through the soil into the plant root and how variation in rhodamine concentration affects the growth and development of the plant. The developmental defects over time are verified by nuclear staining the plant cells. Furthermore, we hypothesis that the chemotropic effect seen in plants guides the root away from rhodamine B high concentration regions to low concentration regions. This will in turn help the plant recover from the injury. These minute structural variations are analyzed with the help of our in-house developed dual-arm multi-level magnification light-sheet microscopy (DMx-LSFM) system. The microscope consists of an automated (translating and rotating) sample holding stage, which helps to observe nearby regions by bringing the region of interest into a plane of focus without disturbing the sample.
Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell’s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.
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