Introducing MD-WISE microscopy, a groundbreaking technique that extends the applications of Photoluminescence (PL) imaging. While PL imaging is widely used for visualizing biological activities, detecting chemical species, and characterizing materials, its effectiveness is limited due to overlapping emission spectra of chromophores. However, MD-WISE overcomes this challenge by utilizing nonlinear interactions between mid-infrared and visible excitations, enabling the differentiation of chromophores with similar spectra in a multi-dimensional space. This is achieved through two mechanisms: modulating the optical absorption cross sections of molecular dyes by exciting specific vibrational groups and reducing the Photoluminescence quantum yield of semiconductor nanocrystals. Unlike most nonlinear optical microscopies that require tightly focused beams, MD-WISE operates under widefield imaging conditions, offering a field of view of 30 by 30 microns. By capturing multi-dimensional information in PL images, MD-WISE microscopy has the potential to revolutionize high-speed widefield imaging by simultaneously tracking numerous species and processes.
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