We report on a new application of laser induced breakdown spectroscopy (LIBS) for the diagnosis of diseases such as
ovarian cancer. We perform detection of ovarian cancer biomarker CA 125 based on LIBS measurements. Immunoconjugated
Silicon particles are incubated with the affinity agarose beads carrying CA125 molecules. In the competitive
affinity method Si particles carrying IgG molecules are pre-incubated with CA125. This pre-incubation decreases the
numbers of free IgG molecules available for consequent interaction with the affinity beads. Thus less Si particles are
attached to the agarose beads and consequently smaller Si peak area is measured by LIBS. We demonstrate a limit-ofdetection
about 30 ppb for model protein avidin. We use two-element coded micro-particles to yield spectroscopic
emission code using LIBS. We show that LIBS-based data collecting technique provides methodology for identification
of biomarkers and cost-effective device for future clinical applications.
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