Prof. Elliot L. Botvinick Profile

Prof. Elliot L. Botvinick

Professor at Univ of California Irvine

SPIE Involvement:

Author

Publications (20)

Proceedings Article | 5 October 2023 Presentation

Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis

Michelle Lanterman, Irene Zhang, Sarah Eldeen, Ulysses Castillo, Andrew Putnam, Elliot Botvinick

Proceedings Volume PC12649, PC126490T (2023) https://doi.org/10.1117/12.2681249

KEYWORDS: Capillaries, Optical tweezers, Matrices, Veins, Time metrology, Lung, Hydrogels, Crosstalk, Confocal microscopy, Bone

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Proceedings Article | 3 August 2021 Presentation

Using light to establish and measure stiffness gradients in three-dimensional engineered tissues

Alicja Jagiello, Qingda Hu, Ulysses Castillo, Elliot Botvinick

Proceedings Volume 11798, 117980A (2021) https://doi.org/10.1117/12.2595590

KEYWORDS: Tissues, Anisotropy, Systems modeling, Optical tweezers, Optical lithography, Optical fibers, Collagen, 3D modeling

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Proceedings Article | 15 March 2018 Open Access

Kinetics of lipid metabolism in cancer cells (Conference Presentation)

Eric Potma, Jue Hou, Elliot Botvinick, Bruce Tromberg

Proceedings Volume 10504, 1050406 (2018) https://doi.org/10.1117/12.2290754

KEYWORDS: Mode conditioning cables, Cancer, Raman scattering, Microscopy, Breast, Breast cancer, Industrial chemicals, Raman spectroscopy, Chemistry, Optical diagnostics

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Proceedings Article | 14 March 2018 Presentation

High-throughput screening of encapsulated islets using wide-field lens-free on-chip imaging (Conference Presentation)

Yibo Zhang, Michael Alexander, Sam Yang, Rahul Krishnan, Yinxu Bian, Elliot Botvinick, Jonathan Lakey, Aydogan Ozcan

Proceedings Volume 10497, 104970T (2018) https://doi.org/10.1117/12.2291453

KEYWORDS: Transplantation, Optical microscopes, Imaging systems, Image sensors, Analytical research, Quality systems, CMOS sensors, Sensors, Blue light emitting diodes, Holograms

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Proceedings Article | 20 February 2018 Paper

Diffusion coefficient of alginate microcapsules used in pancreatic islet transplantation, a method to cure type 1 diabetes

Avid Najdahmadi, Jonathan R. Lakey, Elliot Botvinick

Proceedings Volume 10506, 105061D (2018) https://doi.org/10.1117/12.2318565

KEYWORDS: Diffusion, Transplantation, Polymers, In vivo imaging, Calcium, Ions, Oxygen, Temperature metrology, Particles, Polymerization

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Pancreatic islet transplantation is a promising approach of providing insulin in type 1 diabetes. One strategy to protect islets from the host immune system is encapsulation within a porous biocompatible alginate membrane. This encapsulation provides mechanical support to the cells and allows selective diffusion of oxygen, nutrients and insulin while blocking immunoglobulins. These hydrogels form by diffusion of calcium ions into the polymer network and therefore they are highly sensitive to environmental changes and fluctuations in temperature. We investigated the effects of gel concentration, crosslinking time and ambient conditions on material permeability, volume, and rigidity, all of which may change the immunoisolating characteristics of alginate. To measure diffusion coefficient as a method to capture structural changes we studied the diffusion of fluorescently tagged dextrans of different molecular weight into the midplane of alginate microcapsules, the diffusion coefficient is then calculated by fitting observed fluorescence dynamics to the mathematical solution of 1-D diffusion into a sphere. These measurements were performed after incubation in different conditions as well as after an in vivo experiment in six immunocompetent mice for seven days. Additionally, the changes in gel volume after incubation at different temperatures and environmental conditions as well as changes in compression modulus of alginate gels during crosslinking were investigated. Our result show that increase of polymer concentration and crosslinking time leads to a decrease in volume and increase in compression modulus. Furthermore, we found that samples crosslinked and placed in physiological environment, experience an increase in volume. As expected, these volume changes affect diffusion rates of fluorescent dextrans, where volume expansion is correlated with higher calculated diffusion coefficient. This observation is critical to islet protection since higher permeability due to the expansion in vivo may lead to increased permeability to immunoglobulins. Capsules from the in vivo study showed similar volume expansion and increased permeability, indicating our in vitro assay is a good predictor of volume change in vivo.

SPIE Journal Paper | 27 August 2014 Open Access

Method measuring oxygen tension and transport within subcutaneous devices

John Weidling, Sara Sameni, Jonathan Lakey, Elliot Botvinick

JBO, Vol. 19, Issue 08, 087006, (August 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.8.087006

KEYWORDS: Oxygen, Tissues, In vivo imaging, Mercury, Light emitting diodes, Measurement devices, In vitro testing, Skin, Sensors, Hypoxia

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SPIE Journal Paper | 13 December 2012 Open Access

Lens-free computational imaging of capillary morphogenesis within three-dimensional substrates

John Weidling, Serhan Isikman, Alon Greenbaum, Aydogan Ozcan, Elliot Botvinick

JBO, Vol. 17, Issue 12, 126018, (December 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.12.126018

KEYWORDS: Capillaries, 3D image reconstruction, Holograms, 3D image processing, Microscopy, Holography, Sensors, Digital holography, Computational imaging, Imaging systems

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SPIE Journal Paper | 1 January 2008 Open Access

Use of laser tweezers to analyze sperm motility and mitochondrial membrane potential

Jaclyn Nascimento, Linda Shi, Charlie Chandsawangbhuwana, James Tam, Barbara Durrant, Elliot Botvinick, Michael Berns

JBO, Vol. 13, Issue 01, 014002, (January 2008) https://doi.org/10.1117/12.10.1117/1.2839051

KEYWORDS: Optical tweezers, Luminescence, Optical filters, Biological research, Lanthanum, Microscopes, Phase contrast, Lamps, Video, Robotics

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SPIE Journal Paper | 1 March 2007 Open Access

Laser nanosurgery of single microtubules reveals location-dependent depolymerization rates

Nicole Wakida, Christopher Lee, Elliot Botvinick, Linda Shi, Alexander Dvornikov, Michael Berns

JBO, Vol. 12, Issue 02, 024022, (March 2007) https://doi.org/10.1117/12.10.1117/1.2718920

KEYWORDS: Femtosecond phenomena, Picosecond phenomena, Laser ablation, Control systems, Laser cutting, Luminescence, Laser damage threshold, Ultraviolet radiation, Laser systems engineering, Proteins

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Proceedings Article | 11 September 2006 Paper

Mitotic spindle studied using picosecond laser scissors

N. Baker, E. Botvinick, Linda Shi, M. Berns, George Wu

Proceedings Volume 6326, 63262C (2006) https://doi.org/10.1117/12.680965

KEYWORDS: Spindles, Laser ablation, Microscopes, Picosecond phenomena, Microscopy, Mirrors, Luminescence, Cell death, Ultraviolet radiation, Nd:YAG lasers

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SPIE Journal Paper | 1 September 2006 Open Access

Computer-based tracking of single sperm

Linda Shi, Jaclyn Nascimento, Michael Berns, Elliot Botvinick

JBO, Vol. 11, Issue 05, 054009, (September 2006) https://doi.org/10.1117/12.10.1117/1.2357735

KEYWORDS: Particles, Image segmentation, Detection and tracking algorithms, Computing systems, Video, Phase contrast, Image contrast enhancement, Image enhancement, Optical trapping, Biological research

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SPIE Journal Paper | 1 September 2006 Open Access

Errata: Analysis of sperm motility using optical tweezers

Jaclyn Nascimento, Elliot Botvinick, Linda Shi, Barbara Durrant, Michael Berns

JBO, Vol. 11, Issue 05, 059803, (September 2006) https://doi.org/10.1117/12.10.1117/1.2371122

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Proceedings Article | 30 August 2006 Paper

A real-time single sperm tracking, laser trapping, and ratiometric fluorescent imaging system

Linda Shi, Elliot Botvinick, Jaclyn Nascimento, Charlie Chandsawangbhuwana, Michael Berns

Proceedings Volume 6326, 63260V (2006) https://doi.org/10.1117/12.680912

KEYWORDS: Imaging systems, Computing systems, Luminescence, Optical trapping, Video, Telecommunications, Optical filters, Cameras, Image processing, Optical tweezers

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SPIE Journal Paper | 1 July 2006 Open Access

Analysis of sperm motility using optical tweezers

Jaclyn Nascimento, Elliot Botvinick, Linda Shi, Barbara Durrant, Michael Berns

JBO, Vol. 11, Issue 04, 044001, (July 2006) https://doi.org/10.1117/12.10.1117/1.2337559

KEYWORDS: Optical tweezers, Biological research, Optical trapping, Statistical analysis, Microscopes, Principal component analysis, Optical testing, Video, Polarizers, Lanthanum

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Proceedings Article | 27 August 2005 Paper

Correlation of sperms’ swimming force to their swimming speed as assessed by optical tweezers

Jaclyn Vinson, Elliot Botvinick, Barbara Durrant, Michael Berns

Proceedings Volume 5930, 593026 (2005) https://doi.org/10.1117/12.614505

KEYWORDS: Optical tweezers, Principal component analysis, Video, Cameras, Polarizers, Continuous wave operation, Particles, Microscopes, Objectives, Visible radiation

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Proceedings Article | 27 August 2005 Paper

Axicon-based annular laser trap for studies on sperm activity

Bing Shao, Jaclyn Vinson, Elliot Botvinick, Sadik Esener, Michael Berns

Proceedings Volume 5930, 59300D (2005) https://doi.org/10.1117/12.613116

KEYWORDS: Axicons, Objectives, Optical trapping, Microscopes, Collimation, Optical tweezers, Optical design, Ray tracing, Analytical research, Telescopes

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Proceedings Article | 19 August 2005 Paper

Monitoring sperm mitochondrial respiration response in a laser trap using ratiometric fluorescence

Adrian Mei, Elliot Botvinick, Michael Berns

Proceedings Volume 5930, 59302F (2005) https://doi.org/10.1117/12.618126

KEYWORDS: Luminescence, Acquisition tracking and pointing, Optical tweezers, Particles, LabVIEW, Molecules, Laser scattering, Absorption, Microscopes, Camera shutters

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Proceedings Article | 8 November 2001 Paper

3D quantitative imaging of the microvasculature with the Texas Instruments Digital Micromirror Device

Yeshaiahu Fainman, Elliott Botvinick, Jeffrey Price, David Gough

Proceedings Volume 4457, (2001) https://doi.org/10.1117/12.447746

KEYWORDS: Confocal microscopy, Digital micromirror devices, Mirrors, Microscopes, Micromirrors, 3D image processing, In vivo imaging, Objectives, Stereoscopy, Raster graphics

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Proceedings Article | 27 April 2000 Paper

In-vivo confocal microscopy based on the Texas Instruments digital micromirror device

Elliott Botvinick, Florence Li, Sungdo Cha, David Gough, Yeshaiahu Fainman, Jeffrey Price

Proceedings Volume 3921, (2000) https://doi.org/10.1117/12.384208

KEYWORDS: Confocal microscopy, Mirrors, Digital micromirror devices, Micromirrors, Microscopes, Objectives, Blood, Signal to noise ratio, Luminescence, In vivo imaging

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Proceedings Article | 8 March 1999 Paper

3D profilometry using a dynamically configurable confocal microscope

Sungdo Cha, Paul Lin, Lijun Zhu, Elliott Botvinick, Pang Chen Sun, Yeshaiahu Fainman

Proceedings Volume 3640, (1999) https://doi.org/10.1117/12.341066

KEYWORDS: Confocal microscopy, Digital micromirror devices, Microscopes, Mirrors, 3D image processing, Objectives, 3D image reconstruction, Imaging systems, 3D metrology, Profilometers

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Showing 5 of 20 publications

Conference Committee Involvement (3)

Optical Trapping and Optical Micromanipulation VI

2 August 2009 | San Diego, California, United States

Optical Trapping and Optical Micromanipulation V

10 August 2008 | San Diego, California, United States

Optical Trapping and Optical Micromanipulation IV

26 August 2007 | San Diego, California, United States

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