Digital holographic microscopy (DHM) has been demonstrated to be a suitable label-free and non-invasive quantitative phase imaging (QPI) tool in risk assessment of the cytotoxic potential of engineered nanoparticles and organic nanocarriers. For a broader application, robustness of DHM-based assays needs to be demonstrated towards DHM standardization in risk assessment. Thus, we performed an interlaboratory comparison on the transferability and reproducibility of a DHM-based assay. The cytotoxic potential of organic nanoparticles on A549 lung epithelial cells was analyzed in two European laboratories using identically constructed DHM systems. Our results demonstrate a solid and accurate performance of the DHM-based cytotoxicity assay
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