A fundamental problem of separation of absorption and scattering contributions into light attenuation was resolved using time- and frequency-domain systems. In spite of the progress in the development of time- and frequency-domain hardware, continuous-wave systems offer higher signal-to-noise ratios, better portability, and, potentially, lower equipment and service costs. In biological tissues of known composition one can quantify the chromophore concentrations, such as oxy- and deoxyhemoglobin, water, and fat, and also quantify the reduced scattering coefficient by using broadband approach and by employing characteristic spectral features of chromophores. In this submission we report further developments of our hyperspectral technique to measure absolute concentrations of tissue chromophores.
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