Prof. Hans C. Gerritsen Profile

Prof. Hans C. Gerritsen

Senior lecturer/Professor, Head of section at Utrecht Univ

SPIE Involvement:

Author

Publications (26)

Proceedings Article | 5 March 2015 Paper

Time-resolved spectral imaging: better photon economy, higher accuracy

Farzad Fereidouni, Keimpe Reitsma, Gerhard Blab, Hans Gerritsen

Proceedings Volume 9329, 93290H (2015) https://doi.org/10.1117/12.2080392

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Imaging systems, Luminescence, Imaging spectroscopy, Field programmable gate arrays, Picosecond phenomena, Molecular interactions, Photodetectors, Quantum efficiency

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SPIE Journal Paper | 12 August 2013 Open Access

Blind unmixing of spectrally resolved lifetime images

Farzad Fereidouni, Gerhard Blab, Hans Gerritsen

JBO, Vol. 18, Issue 08, 086006, (August 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.8.086006

KEYWORDS: Image segmentation, Luminescence, Visualization, Clouds, Superposition, Binary data, RGB color model, Image analysis, Photons, Picosecond phenomena

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SPIE Journal Paper | 19 November 2012 Open Access

Carcinogenic damage to deoxyribonucleic acid is induced by near-infrared laser pulses in multiphoton microscopy via combination of two- and three-photon absorption

Oleg Nadiarnykh, Giju Thomas, Johan van Voskuilen, Henricus Sterenborg, Hans Gerritsen

JBO, Vol. 17, Issue 11, 116024, (November 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.11.116024

KEYWORDS: Absorption, In vivo imaging, Luminescence, Tissues, Nonlinear optics, Multiphoton microscopy, Femtosecond phenomena, Ultraviolet radiation, Photons, Harmonic generation

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Proceedings Article | 4 May 2012 Paper

Data driven identification and aberration correction for model-based sensorless adaptive optics

J. Antonello, R. Fraanje, H. Song, M. Verhaegen, H. Gerritsen, C. Keller, T. van Werkhoven

Proceedings Volume 8436, 84360S (2012) https://doi.org/10.1117/12.922002

KEYWORDS: Aberration correction, Deformable mirrors, Data modeling, Adaptive optics, Sensors, Wavefronts, Photodiodes, Imaging systems, Modeling, Radon

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Proceedings Article | 16 February 2012 Paper

Coherence-gated wavefront sensing for microscopy using fringe analysis

Tim van Werkhoven, Hoa Truong, Jacopo Antonello, Rufus Fraanje, Hans Gerritsen, Michel Verhaegen, Christoph Keller

Proceedings Volume 8253, 82530E (2012) https://doi.org/10.1117/12.905797

KEYWORDS: Fringe analysis, Wavefront sensors, Microscopy, Microscopes, Wavefronts, Microelectromechanical systems, Adaptive optics, Current controlled current source, Cameras, Frequency modulation

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SPIE Journal Paper | 1 July 2008 Open Access

Design and implementation of a sensitive high-resolution nonlinear spectral imaging microscope

Jonathan Palero, Gwendal Latouche, Henriette Bruijn, Angélique van der Ploeg-van den Heuvel, Henricus Sterenborg, Hans Gerritsen

JBO, Vol. 13, Issue 04, 044019, (July 2008) https://doi.org/10.1117/12.10.1117/1.2953180

KEYWORDS: Imaging spectroscopy, Tissues, Imaging systems, Skin, Second-harmonic generation, Microscopes, Luminescence, Objectives, Spectral resolution, Nonlinear optics

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Proceedings Article | 19 February 2007 Paper

Confocal time-resolved fluorescence anisotropy imaging

Arjen Bader, Erik Hofman, Paul van Bergen en Henegouwen, Hans Gerritsen

Proceedings Volume 6441, 64410C (2007) https://doi.org/10.1117/12.702031

KEYWORDS: Anisotropy, Fluorescence anisotropy, Confocal microscopy, Time resolved spectroscopy, Fluorescence lifetime imaging, Polarization, Single photon, Plasma, Luminescence, Microscopes

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Proceedings Article | 10 February 2007 Paper

Spectrally resolved multiphoton imaging of post-mortem biopsy and in-vivo mouse skin tissues

Jonathan Palero, Henriëtte de Bruijn, Angélique van der Ploeg van den Heuvel, Henricus Sterenborg, Hans Gerritsen

Proceedings Volume 6442, 64421B (2007) https://doi.org/10.1117/12.702059

KEYWORDS: Tissues, Skin, In vivo imaging, Collagen, Luminescence, Biopsy, Second-harmonic generation, Microscopy, Multiphoton microscopy, Imaging spectroscopy

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Proceedings Article | 14 April 2006 Paper

Three-dimensional multiphoton autofluorescence spectral imaging of live tissues

Jonathan Palero, Henriëtte de Bruijn, Angélique van der Ploeg van den Heuvel, Henricus Sterenborg, Hans Gerritsen

Proceedings Volume 6191, 61910J (2006) https://doi.org/10.1117/12.663736

KEYWORDS: Tissues, Skin, Imaging spectroscopy, Collagen, Second-harmonic generation, Luminescence, Microscopy, Imaging systems, Optical fibers, Tissue optics

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Proceedings Article | 23 February 2006 Paper

Photonic crystal fiber as a tunable light source for visible wavelength two-photon microscopy

Jonathan Palero, Vincent Boer, Jacob Vijverberg, Henricus Sterenborg, Hans Gerritsen

Proceedings Volume 6089, 60890T (2006) https://doi.org/10.1117/12.642248

KEYWORDS: Visible radiation, Luminescence, Photonic crystal fibers, Solitons, Picosecond phenomena, Microscopy, Light sources, Two photon excitation microscopy, Dispersion, Microscopes

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Proceedings Article | 23 February 2006 Paper

In vivo intrinsic emission spectral imaging microscopy of mouse skin tissues

Jonathan Palero, Henriëtte de Bruijn, Angélique van der Ploeg van den Heuvel, Henricus Sterenborg, Hans Gerritsen

Proceedings Volume 6089, 60891A (2006) https://doi.org/10.1117/12.642239

KEYWORDS: Tissues, Skin, Imaging spectroscopy, Luminescence, Microscopy, Collagen, Multiphoton microscopy, In vivo imaging, Spectroscopy, Image visualization

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Proceedings Article | 23 February 2006 Paper

Two-photon spectral imaging microscopy of skin tissues

Jonathan Palero, Henriëtte de Bruijn, Henricus Sterenborg, Hans Gerritsen

Proceedings Volume 6089, 608908 (2006) https://doi.org/10.1117/12.642254

KEYWORDS: Tissues, Imaging spectroscopy, Skin, Second-harmonic generation, Microscopy, Spectroscopy, Charge-coupled devices, RGB color model, Microscopes, Luminescence

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Proceedings Article | 26 October 2005 Paper

Non-linear microscopy and spectroscopy of skin tissues

Jonathan Palero, Gwendal Latouche, Henriëtte de Bruijn, Hans Gerritsen, Henricus Sterenborg

Proceedings Volume 5968, 59680H (2005) https://doi.org/10.1117/12.660069

KEYWORDS: Skin, Tissues, Second-harmonic generation, Luminescence, Microscopy, Collagen, Charge-coupled devices, Spectrographs, Microscopes, Prisms

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SPIE Journal Paper | 1 November 2004 Open Access

Fast fluorescence lifetime imaging of calcium in living cells

Alexandra Agronskaia, L. Tertoolen, Hans Gerritsen

JBO, Vol. 9, Issue 06, (November 2004) https://doi.org/10.1117/12.10.1117/1.1806472

KEYWORDS: Calcium, Luminescence, Fluorescence lifetime imaging, Calibration, Pulsed laser operation, Microscopes, Sensors, Image intensifiers, Imaging systems, Ions

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Proceedings Article | 21 June 2004 Paper

High-speed fluorescence lifetime imaging

Hans Gerritsen, Dave van den Heuvel, Alexandra Agronskaia

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.529582

KEYWORDS: Luminescence, Sensors, Fluorescence lifetime imaging, Photons, Molecules, Electronics, Picosecond phenomena, Gallium arsenide, Microscopes, Single photon

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Proceedings Article | 21 June 2002 Paper

Lifetime imaging of the vital DNA/RNA probe SYTO13 in healthy and apoptotic cells

Marc van Zandvoort, Wim Engels, Cees de Grauw, Hans Gerritsen, Dick Slaaf

Proceedings Volume 4626, (2002) https://doi.org/10.1117/12.472113

KEYWORDS: Luminescence, Cell death, Absorbance, In vitro testing, Mass attenuation coefficient, Quantum efficiency, Fluorescence lifetime imaging, Fluorescence spectroscopy, Mirrors, Refractive index

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Proceedings Article | 17 June 2002 Paper

FRET imaging microscopy

Brian Herman, Mao Sun, Atsushi Masuda, Hans Gerritsen, Victoria Centonze

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470683

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Luminescence, Cell death, Microscopy, Microscopes, Multiphoton microscopy, Energy transfer, Electronic filtering, Fluorescence lifetime imaging

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Proceedings Article | 10 May 2001 Paper

Fluorescence lifetime imaging module LIMO for CLSM

Cornelius van der Oord, Cees de Grauw, Hans Gerritsen

Proceedings Volume 4252, (2001) https://doi.org/10.1117/12.426732

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Bragg cells, Pulsed laser operation, Microscopes, Continuous wave operation, Sensors, Ions, Molecules, Electronics

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Proceedings Article | 24 April 2001 Paper

Two-photon lifetime imaging of blood and blood vessels

Cees de Grauw, Marc van Zandvoort, M. oude Egbrink, Dick Slaaf, Hans Gerritsen

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424551

KEYWORDS: Blood, Luminescence, Blood vessels, Two photon excitation microscopy, Fluorescence lifetime imaging, Microscopes, Microscopy, Absorption, Two photon imaging, Molecules

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Proceedings Article | 18 December 2000 Paper

Fluorescence lifetime imaging of oxygen in dental biofilm

Hans Gerritsen, Cees de Grauw

Proceedings Volume 4164, (2000) https://doi.org/10.1117/12.410635

KEYWORDS: Oxygen, Luminescence, Fluorescence lifetime imaging, Ruthenium, Bacteria, Calibration, Ions, Microscopy, Molecules, Multiphoton microscopy

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Proceedings Article | 23 March 1995 Paper

Development of a confocal laser scanning fluorescence microscope using two-photon excitation in combination with time-gated detection

Joost Sytsma, Jurrien Vroom, Hans Gerritsen, Yehudi Levine

Proceedings Volume 2412, (1995) https://doi.org/10.1117/12.205328

KEYWORDS: Luminescence, Microscopes, Confocal microscopy, Laser scanners, Objectives, Photons, Near infrared, Molecules, Absorption, 3D image processing

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Proceedings Article | 1 February 1995 Paper

Confocal fluorescence lifetime imaging of ion concentrations

Hans Gerritsen, Renata Sanders, Arie Draaijer

Proceedings Volume 2329, (1995) https://doi.org/10.1117/12.200890

KEYWORDS: Ions, Luminescence, Calibration, Fluorescence lifetime imaging, Calcium, In vivo imaging, Confocal microscopy, Microscopes, In vitro testing, Error analysis

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Proceedings Article | 17 August 1994 Paper

Fluorescence imaging and time-resolved spectroscopy of steroid using confocal synchrotron radiation microscopy

Hans Gerritsen, C. van der Oord, Yehudi Levine, Ian Munro, Gareth Jones, D. Shaw, Fokko Rommerts

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182732

KEYWORDS: Luminescence, Confocal microscopy, Synchrotron radiation, Microscopes, Time resolved spectroscopy, Ultraviolet radiation, Imaging spectroscopy, Absorption, Microscopy, Molecules

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Proceedings Article | 17 August 1994 Paper

Confocal fluorescence lifetime imaging of pH in single cells

Renata Sanders, Hans Gerritsen, Arie Draaijer, P. Houpt, Sjaak van Veen, Yehudi Levine

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182761

KEYWORDS: Luminescence, Confocal microscopy, Fluorescence lifetime imaging, Calibration, Microscopes, Proteins, Microscopy, Molecules, Astatine, Biophysics

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Proceedings Article | 1 April 1992 Paper

Synchrotron radiation as a light source in confocal microscopy of biological processes

Hans Gerritsen, C. van der Oord, Yehudi Levine, Ian Munro, Wendy Myring, D. Shaw, Fokko Rommerts

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58271

KEYWORDS: Microscopes, Synchrotron radiation, Confocal microscopy, Luminescence, Ultraviolet radiation, Mirrors, Light sources, Biochemistry, Beam splitters, Objectives

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Proceedings Article | 1 April 1992 Paper

Determination of the orientation of fluorescent labels relative to myosin S1 in solution from time-resolved fluorescence anisotropy experiments

Uulke van der Heide, Hans Gerritsen, Ian Trayer, Yehudi Levine

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58265

KEYWORDS: Proteins, Molecules, Fluorescence anisotropy, Luminescence, Motion models, Data modeling, Phosphorescence, Absorption, Laser spectroscopy, Biochemistry

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The time-resolved fluorescence anisotropy of myosin S₁ covalently labeled with Eosin-5- maleimide and 1,5-I-AEDANS was measured in solution. Each probe was specifically attached at one SH-group on the S1. The two most reactive SH sites on the heavy chain of the myosin S₁ were used. The fluorescence anisotropy was measured at different excitation wavelengths. In this way, several absorption moments were utilized, each having a distinct orientation in the frame of the dye. The orientations of the transition moments in the dyes were determined in a separate experiment using an angle resolved fluorescence depolarization experiment on dyes embedded in stretched matrices of PVA polymers. The anisotropy decay curves exhibit fast (<3 ns) and slow (> 100 ns) components. The slow decay components reflect the motion of the large protein molecules. The fast anisotropy decay are attributed to a fast, but restricted, motion of the bound dye relative to the protein as experiments on free dyes in solution reveal subnanosecond anisotropy decays. The anisotropy decays have been analyzed in terms of a model which describes the restricted motion of the dye molecule relative to the protein and the overall rotation of the dye-protein complex in solution. An important element in the model is the incorporation of the orientational distribution of the dye relative to the protein. The observed anisotropy decays were analyzed using a global target approach in which the experimental data obtained at different excitation wavelengths are fitted simultaneously to the theoretical model. It is important to note that the orientational distribution of the dye relative to the protein, as well as the rotational correlation times of the motions for a dye attached to a given binding site, are independent of the excitation wavelength used. This leads to a reduction in the number of independent parameters optimized by the nonlinear least squares procedure. The orientational distribution of the dye relative to the protein obtained in this way is particularly useful for the interpretation of fluorescence depolarization data obtained from labeled muscle fibers. Indeed, knowledge of the distribution function of a dye attached to a binding site of the S1 protein is a prerequisite for a probe-independent determination of the orientational distribution of the S₁ proteins themselves in the muscle fiber.

Showing 5 of 26 publications

Conference Committee Involvement (16)

Multiphoton Microscopy in the Biomedical Sciences XVII

29 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVI

14 February 2016 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XV

8 February 2015 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIV

2 February 2014 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIII

3 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XII

22 January 2012 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XI

23 January 2011 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences X

24 January 2010 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences IX

25 January 2009 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences VIII

20 January 2008 | San Jose, California, United States

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

17 June 2007 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences VII

21 January 2007 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences VI

22 January 2006 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences V

23 January 2005 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences IV

25 January 2004 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences III

26 January 2003 | San Jose, CA, United States

Showing 5 of 16 Conference Committees

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