The parameter information of particle size and position in particle field is of great significance in scientific research, engineering and other fields. In this paper, after the particle hologram is obtained by in-line digital holography, the angular spectrum algorithm is used to reconstruct the particle field, and the amplitude and phase information of the particle field are obtained; The gray gradient compound method is used for particle recognition, and the particles are separated from the background; Then the watershed segmentation algorithm is used to segment the overlapping particles, and the Hough transform is used to extract the particle size, transverse position and other information; Finally, the depth information of particles is determined by Laplace operator function method, and the three-dimensional field distribution of particle field is obtained. The experimental results show that the measurement error of polystyrene particles with standard diameter of 50μm is 1.53μm and the measurement error rate is 3.06%, which proves the feasibility of this method.
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