We have investigated the enhanced Raman spectra of AMR bacteria strains of E. coli using silver coated silicon nanowires SERS assay. Three different E. coli strains, E. coli CCUG17620, NCTC 13441, and A239, were detected using two different excitation laser wavelengths. We found stable and enhanced SERS spectrum using 785 nm laser as opposed to 532 nm. Future development of SERS-chip could offer a reliable platform for direct identification of the pathogen in bio-fluid samples at strains level.
Motility of cells plays an important role to determine the cell growth, health and to monitor the gene transformation. The aim of the current study is to propose a combination of a high-contrast label-free imaging method and a computational approach (conventionally used for super-resolution) which can be used as a tool in tracing the motion of the cells and organelles. Here, we integrate quantitative phase microscopy (QPM) with waveguide-based trapping (WT) and applied multiple signal classification algorithm (MUSICAL) to analyze the motion of the trapped particle. We successfully trapped and displaced a 1 µm polystyrene bead particle on a strip waveguide using a 1064 nm laser beam. While propelling the polystyrene bead particle, we recorded time-lapsed interferometric images using a partially spatially coherent (PSC) light-based off-axis QPM system. The reconstruction of time-lapsed phase images of the trapped particle is accomplished using the Fourier transform and transport of intensity algorithm, which further used in MUSICAL for the motion trace analysis. Here, we traced the motion of a trapped bead particle with scale finer than the size of the object i.e., diffraction limit of the system. We show super-resolved motion trace even though the particle’s image is itself diffraction limited in each frame. The proposed study could be useful in different biological applications such as cell monitoring, cell tracking, manipulation, and classification between healthy and unhealthy cells.
In this project it was found that Fourier ptychographic microscopy can be improved far beyond its conventional limits via waveguide-based optical systems. Extensive in silico studies showed that images obtained on high-refractive index material waveguide chips in conjunction with hyperspectral illumination light and finely designed waveguide geometries can be combined via a modified phase-retrieval algorithm to yield a resolution below 150 nm.
The evanescent field from an optical waveguide is used for near-field trapping and transporting of fluorescent microspheres. Out-of-focus fluorescence imaging is used to track the trapped particle in 3-D with nanometer precision (<100 nm). A prior calibration is done to determine the relationship between the z-coordinate and the radius of the outermost diffraction ring in the image of the sphere. This gives precise information about how much the particle moves up and down during propulsion along the waveguide. Results are presented for trapping and tracking a 1 μm fluorescent particle on a strip waveguide.
KEYWORDS: Particles, Nanoparticles, Gold, Glasses, Near field scanning optical microscopy, Near field optics, Microscopes, Nanostructuring, Optical tweezers, Objectives
We propose to optically trap nanoparticles utilizing a single nanostructured glass-fiber tip. 3D translation of optically trapped nanoparticles - nano tweezers - presents vast application possibilities and has not yet been shown. The input end of the fibre probe is a standard fibre, providing easy coupling to a light source. The output end is tapered down and covered with gold, with a nanoaperture fabricated on the tip. The nanoaperture provides the strong field gradient necessary for trapping of nanoparticles. We discuss probe geometries supported by numerical simulations. The fabrication procedure for the fibre probe, using a focused ion beam, is described. A set-up for the experiments has been made and preliminary trapping results are presented.
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