We present mesoscale imaging of nanoparticle-labeled cells in 3D culture with photothermal optical coherence tomography (PT-OCT). Cells labeled with gold nanorods (AuNRs) are encapsulated in agarose-based gel for PT-OCT with 660-nm laser. We show a binary PT contrast for single cell detection without causing cell death. We also demonstrate 3D mapping of AuNR-labeled cells within optically scattering media by imaging through a layer of polystyrene beads mimicking highly scattering tissue and through a layer of cells without AuNRs. This method has an imaging scale that complements the other major cell imaging techniques for studying live cells in 3D culture.
The unique 3D imaging scale of OCT enables high-resolution tracking of large-size particle over a millimeter-level field of view. However, limited volumetric imaging speed hinders the assessment of fast movement, where a significant sacrifice in spatial sampling or transverse scanning distance is often needed. To address this technical hurdle in the application of studying the transport of preimplantation embryos in the mouse oviduct (fallopian tube) in vivo, we developed a new velocimetry method that relies on the particle streak formed in a single OCT volume containing double B-scans at each B-scan location, providing 3D structure information with particle velocity.
A hollow porous platinum nanostructure was synthesized using a novel silver-micelle combined dual template method. Such nanoparticles enabled photothermal therapy of cancers with near-infrared second window (NIR-II). Because of the unique hollow core and porous shell structure, drastically increased loading capacity was achieved with anti-tumor therapeutics. Furthermore, the high catalysis efficiency of platinum porous shell could effectively catalyze the decomposition of endogenous H2O2 into O2 to relieve the tumorous hypoxia microenvironment and consequently enhance the therapeutic efficiency. Apparently, the hollow porous platinum nanostructures endow the possibility of treating tumors with multimodal imaging-guided NIR-II photothermal therapy in combination with other therapies.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.