A Raman spectroscopic investigation of a genetically altered Human Embryonic Kidney Cell (HEK293) along with a
pathologically normal cell has been carried out by a conventional method. The genetic alteration was carried out with a
standard protocol by using a Green Fluorescence Protein (GFP). Raman spectra show that there are dramatic differences
between the spectrum obtained from a genetically altered cell and that obtained from a pathologically normal cell. The
former shows three broad bands; meanwhile the latter shows several sharp peaks corresponding to the ring vibrational
modes of Phen, GFP and DNA. The present analysis provides an indication that the force field near Phen located at 64,
65 and 66 was altered during the genetic transformation. The Raman spectrum could be a direct experimental evidence
for substantial modifications triggered due to the expression of specific genes.
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