Epivascular microstructures, a potential biomarker for retinal diseases, are investigated in the living human retina using adaptive optics optical coherence tomography (AO-OCT). The AO correction is driven by a four-sided pyramid wavefront sensor with a loop bandwidth of 30 Hz. In order to achieve a stable placement of the focus of the imaging beam in the desired retinal layer, a new concept for focus shifting is introduced which uses an in vivo calibration routine that is performed pre-imaging in each subject. The capability of the instrument is demonstrated by visualizing hyporeflective microstructures situated along the retinal vasculature with single volume AO-OCT images recorded at an extended 4° x 4° field of view.
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