Cross-grating phase microscopy (CGM) is a wavefront imaging technique that can map the intensity and phase of a light beam with high sensitivity and spatial resolution. CGM is based on the association of a 2D-grating with a camera, separated by a millimetric distance. In this contribution, we will show how CGM can measure the dry mass of single neurites of neural cells in culture, and how it can highlight the subtle dynamics and mass transport within neurites, in a label-free manner.
Recently, we introduced a novel treatment of Alzheimer's disease (AD) consisting of the use of photobiomodulation (PBM) coupled with a static magnetic field . The efficiency of this approach was evidenced on a model of mice affected by a neurotoxic agent (Amyloid-β(25-35)).
In this communication, we will present recent results using this approach at the scale of neural cells in culture, as an attempt to enhance the benefit of light starting from a much simpler model than an entire mammalian organism. We will highlight the interest of using imaging instead of ensemble fluorescence measurements, and comment on parameters such as the PBM wavelength, fluence and glutamate concentration.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.