We present a compact and portable stimulated Raman scattering (SRS) imaging system capable of high speed, label-free imaging of cells and tissues. Our setup rapidly acquires multispectral datasets with high chemical specificity by scanning the spectral range of 700-3100 1/cm in just 100 ms, providing a tenfold increase in acquisition speed. It allowed to visualize cell nucleus and cytoplasm changes during drug induced cancer cell death. SRS offers distinct advantages, providing valuable insights into drug-cell interactions, cell morphology, and chemical changes without the interference of exogenous labels. The novel high-speed SRS systems allows rapid screening of drug effects on cancer cells.
We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum during tumor surgeries. Leveraging the advantages of a compact and robust fiber laser, we have integrated the entire microscopy system into a clinical cart, facilitating deployment in diverse clinical environments. The laser provides rapid tunability within milliseconds across a broad spectral range of 700 to 3300 cm^-1, covering biomedically relevant resonances in the fingerprint region. For detailed examination of larger tissue samples, we have designed a high-speed, low-resolution imaging mode to quickly identify cancerous hot-spots, followed by a high-resolution imaging mode.
We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum during tumor surgeries. Leveraging the advantages of a compact and robust fiber laser, we have integrated the entire microscopy system into a clinical cart, facilitating deployment in diverse clinical environments. The laser provides rapid tunability within milliseconds across a broad spectral range of 700 to 3300 cm^-1, covering biomedically relevant resonances in the fingerprint region. For detailed examination of larger tissue samples, we have designed a high-speed, low-resolution imaging mode to quickly identify cancerous hot-spots, followed by a high-resolution imaging mode.
Hyperspectral Stimulated Raman Imaging (SRS) has shown great promise as a label-free chemical imaging technique in biomedical and medical research. We present recent developments in SRS integrating a compact and portable all-fiber laser with balanced detection into an imaging system, aiming to enhance ease-of-use, specificity, and reliability in acquiring high-speed, multicolor chemical images. The system's adaptability is highlighted by integrating the entire microscopy system into a clinical cart, ensuring clinical compatibility as well as its seamless integration with a Nikon Eclipse Ti widefield microscope, providing a compact and robust extension for varied imaging setups. The system incorporates a balanced detector to enable shot-noise-limited measurements, accommodating over 100mW of Stokes power on the detector.
We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum during tumor surgeries. Leveraging the advantages of a compact and robust fiber laser, we have integrated the entire microscopy system into a clinical cart, facilitating deployment in diverse clinical environments. The laser provides rapid tunability within milliseconds across a broad spectral range of 700 to 3300 cm^-1, covering biomedically relevant resonances in the fingerprint region. For detailed examination of larger tissue samples, we have designed a high-speed, low-resolution imaging mode to quickly identify cancerous hot-spots, followed by a high-resolution imaging mode.
Hyperspectral Stimulated Raman Imaging (SRS) has shown great promise as a label-free chemical imaging technique in biomedical and medical research. We present recent developments in SRS integrating a compact and portable all-fiber laser with balanced detection into an imaging system, aiming to enhance ease-of-use, specificity, and reliability in acquiring high-speed, multicolor chemical images. The system's adaptability is highlighted by its seamless integration with a Nikon Eclipse Ti widefield microscope, providing a compact and robust extension for varied imaging setups. The system incorporates a balanced detector to enable shot-noise-limited measurements, accommodating over 100mW of Stokes power on the detector.
We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum during tumor surgeries. For detailed examination of larger tissue samples, we have designed a high-speed, low-resolution imaging mode to quickly identify cancerous hot-spots, followed by a high-resolution imaging mode. Leveraging the advantages of a compact and robust fiber laser, we have integrated the entire microscopy system into a clinical cart, facilitating deployment in diverse clinical environments. The laser provides rapid tunability within milliseconds across a broad spectral range of 700 to 3300 1/cm, covering biomedically relevant resonances in the fingerprint region.
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