Ms. Noelle Daigle Profile

Noelle Daigle

at Wyant College of Optical Sciences

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Publications (6)

Proceedings Article | 13 March 2024 Presentation

Multiphoton microscopy combined with machine learning shows promise for localizing pancreatic neuroendocrine tumors

Noelle Daigle, Suzann Duan, Juanita Merchant, Travis Sawyer

Proceedings Volume PC12846, PC1284607 (2024) https://doi.org/10.1117/12.3000595

KEYWORDS: Biological samples, Tumors, Tissues, Multiphoton microscopy, Machine learning, Education and training, Statistical modeling, Image classification, Algorithm development, Visualization

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Proceedings Article | 12 March 2024 Presentation + Paper

Evaluating the impact of freeze-thaw protocols on tissue microstructural imaging features measured using optical coherence tomography

Mackenna Kropatsch, Noelle Daigle, Suzann Duan, Ricky Sontz, Juanita Merchant, Travis Sawyer

Proceedings Volume 12854, 1285404 (2024) https://doi.org/10.1117/12.3001355

KEYWORDS: Tissues, Optical coherence tomography, Liver, Lung, Biological imaging, Biological samples, 3D microstructuring, Statistical analysis, Optical testing, Nitrogen

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Proceedings Article | 12 March 2024 Presentation + Paper

Automated classification of pancreatic neuroendocrine tumors using label-free multiphoton microscopy and deep learning

Shuyuan Guan, Noelle Daigle, Travis Sawyer

Proceedings Volume 12854, 128540B (2024) https://doi.org/10.1117/12.3018502

KEYWORDS: Education and training, Tumors, Image classification, Deep learning, Data modeling, Multiphoton microscopy, Machine learning, Second harmonic generation, RGB color model, Tissues

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SPIE Journal Paper | 23 January 2024 Open Access

Combined flat-field and frequency filter approach to correcting artifacts of multichannel two-photon microscopy

Thomas Knapp, Natzem Lima, Noelle Daigle, Suzann Duan, Juanita L. Merchant, Travis Sawyer

JBO, Vol. 29, Issue 01, 016007, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.1.016007

KEYWORDS: Image processing, Tunable filters, Tissues, Image filtering, Image quality, Vignetting, Image fusion, Biological samples, Fourier transforms, Autofluorescence

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SPIE Journal Paper | 13 September 2023 Open Access

Wide field-of-view fluorescence imaging for organ-level lineage tracing of rare intestinal stem cell populations

Noelle Daigle, Suzann Duan, Heyu Song, Natzem Lima, Ricky Sontz, Juanita L. Merchant, Travis W. Sawyer

JBO, Vol. 28, Issue 09, 096004, (September 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.9.096004

KEYWORDS: Tissues, Fluorescence imaging, Stem cells, Animal model studies, Fluorescence, Intestine, Fluorescence microscopy, Proteins, Animals, Light sources and illumination

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SignificanceLineage tracing using fluorescent reporters is a common tool for monitoring the expression of genes and transcription factors in stem cell populations and their progeny. The zinc-binding protein 89 (ZBP-89/Zfp148 mouse gene) is a transcription factor that plays a role in gastrointestinal (GI) stem cell maintenance and cellular differentiation and has been linked to the progression of colon cancer. While lineage tracing is a useful tool, it is commonly performed with high-magnification microscopy on a small field of view within tissue sections, thereby limiting the ability to resolve reporter expression at the organ level. Furthermore, this technique requires extensive tissue processing, which is time consuming and requires euthanizing the animal. Further knowledge could be elucidated by measuring the expression of fluorescent reporters across entire organs with minimal tissue processing.AimWe present the application of wide-field fluorescence imaging for whole-organ lineage tracing of an inducible Zfp148-tdTomato-expressing transgenic mouse line to assess the expression of ZBP-89/Zfp148 in the GI tract.ApproachWe measured tdTomato fluorescence in ex vivo organs at time points between 24 h and 6 months post-induction. Fluctuations in tdTomato expression were validated by fluorescence microscopy of tissue sections.ResultsQuantification of the wide field-of-view images showed a statistically significant increase in fluorescent signal across the GI tract between transgenic mice and littermate controls. The results also showed a gradient of decreasing reporter expression from proximal to distal intestine, suggesting a higher abundance of ZBP-89 expressing stem cells, or higher expression of ZBP-89 within the stem cells, in the proximal intestine.ConclusionsWe demonstrate that wide-field fluorescence imaging is a valuable tool for monitoring whole-organ expression of fluorescent reporters. This technique could potentially be applied in vivo for longitudinal assessment of a single animal, further enhancing our ability to resolve rare stem cell lineages spatially and temporally.

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Proceedings Article | 6 March 2023 Poster + Paper

Combined multiphoton microscopy and somatostatin receptor type 2 imaging of pancreatic neuroendocrine tumors

Noelle Daigle, Thomas Knapp, Suzann Duan, David Jones, Ali Azhdarinia, Sukhen Ghosh, Solmaz AghaAmiri, Naruhiko Ikoma, Jeannelyn Estrella, Martin Schnermann, Juanita Merchant, Travis Sawyer

Proceedings Volume 12371, 1237109 (2023) https://doi.org/10.1117/12.2648113

KEYWORDS: Tumors, Biological samples, Tissues, Second harmonic generation, Multiphoton microscopy, Fluorescence, Autofluorescence, Cancer, Surgery, Emission wavelengths

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