Ms. Paola L. Alacron Profile

Paola L. Alacron

at Univ of Massachusetts Boston

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Publications (4)

SPIE Journal Paper | 11 April 2020 Open Access

Clinical evaluation of smartphone-based fluorescence imaging for guidance and monitoring of ALA-PDT treatment of early oral cancer

Shakir Khan, M. A. Bilal Hussain, Amjad Khan, Hui Liu, Shaista Siddiqui, Srivalleesha Mallidi, Paola Alacron, Liam Daly, Grant Rudd, Filip Cuckov, Colin Hopper, Stephen Bown, Kafil Akhtar, Syed Abrar Hasan, Shahid Ali Siddiqui, Tayyaba Hasan, Jonathan Celli

JBO, Vol. 25, Issue 06, 063813, (April 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.6.063813

KEYWORDS: Luminescence, Image segmentation, Photodynamic therapy, RGB color model, Cancer, Oncology, Imaging devices, Image analysis, Statistical analysis, Phototherapy

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Significance: India has one of the highest rates of oral cancer incidence in the world, accounting for 30% of reported cancers. In rural areas, a lack of adequate medical infrastructure contributes to unchecked disease progression and dismal mortality rates. Photodynamic therapy (PDT) has emerged as an effective modality with potential for treating early stage disease in resource-limited settings, while photosensitizer fluorescence can be leveraged for treatment guidance. Aim: Our aim was to assess the capability of a simple smartphone-based device for imaging 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence for treatment guidance and monitoring as part of an ongoing clinical study evaluating low-cost technology for ALA-based PDT treatment of early oral cancer. Approach: A total of 29 subjects with <2 cm diameter moderately/well-differentiated microinvasive ( < 5 mm depth) oral squamous cell carcinoma lesions (33 lesions total, mean area ∼1.23 cm2) were administered 60 mg / kg ALA in oral solution and imaged before and after delivery of 100 J / cm2 total light dose to the lesion surface. Smartphone-based fluorescence and white light (WL) images were analyzed and compared with ultrasound (US) imaging of the same lesions. Results: We present a comparative analysis of pre- and post-treatment fluorescence, WL, and US images of oral lesions. There was no significant difference in the distribution of lesion widths measured by fluorescence and US (mean widths of 14.5 and 15.3 mm, respectively) and linear regression shows good agreement (R2 = 0.91). In general, PpIX fluorescence images obtained prior to therapeutic light delivery are able to resolve lesion margins while dramatic photobleaching (∼42 % ) is visible post-treatment. Segmentation of the photobleached area confirms the boundaries of the irradiated zone. Conclusions: A simple smartphone-based approach for imaging oral lesions is shown to agree in most cases with US, suggesting that this approach may be a useful tool to aid in PDT treatment guidance and monitoring photobleaching as part of a low-cost platform for intraoral PDT.

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Proceedings Article | 14 August 2019 Presentation

Photodynamic therapy as an alternative treatment modality for early stage buccal mucosa malignancy (Conference Presentation)

Shahid Ali Siddiqui, M. A. Bilal Hussain, Amjad P. Khan, Kafil Akhtar, Shaista Siddiqui, Shakir Khan, Hui Liu, Srivalleesha Mallidi, Grant Rudd, Liam Daly, Paola L. Alacron, Filip Cuckov, Satish C. Sharma, Ibne Ahmed, Stephen G. Bown, Colin Hopper, Jonathan P. Celli, Syed Abrar Hasan, Tayyaba Hasan

Proceedings Volume 11070, 1107053 (2019) https://doi.org/10.1117/12.2525686

KEYWORDS: Photodynamic therapy, Cancer, Oncology, Surgery, Radiotherapy, Light emitting diodes, Light sources, 3D image processing, Light, Luminescence

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Proceedings Article | 14 August 2019 Presentation

Interpretation of ultrasonography indicators of photodynamic therapy treatment response in early malignancy of buccal mucosa (Conference Presentation)

Shaista Siddiqui, Ibne Ahmed, Kafil Akhtar, Shahid Ali Siddiqui, M. A. Bilal Hussain, Shakir Khan, Srivalleesha Mallidi, Amjad P. Khan, Hui Liu, Filip Cuckov, Grant Rudd, Liam Daly, Colin Hopper, Stephen G. Bown, Satish C. Sharma, Syed Abrar Hasan, Jonathan P. Celli, Tayyaba Hasan, Paola Leon

Proceedings Volume 11070, 110703Y (2019) https://doi.org/10.1117/12.2525982

KEYWORDS: Photodynamic therapy, Ultrasonography, Biopsy, Cancer, Biological research, Light emitting diodes, Light sources, Inflammation

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Proceedings Article | 12 August 2019 Presentation + Paper

Clinical evaluation of smartphone-based fluorescence imaging for guidance and monitoring of ALA PDT

Shakir Khan, M. A. Bilal Hussain, Amjad Khan, Hui Liu, Shaista Siddiqui, Srivalleesha Mallidi, Paola Leon, Liam Daly, Grant Rudd, Filip Cuckov, Colin Hopper, Stephen Bown, Shahid Siddiqui, Jonathan Celli, Tayyaba Hasan

Proceedings Volume 11070, 1107059 (2019) https://doi.org/10.1117/12.2526116

KEYWORDS: Luminescence, Photodynamic therapy, Cancer, Tissue optics, Image segmentation, Light emitting diodes, Imaging devices, Light, RGB color model, Phototherapy

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