Ms. Patcharaporn Tippayawat Profile

Patcharaporn Tippayawat

at Khon Kaen Univ.

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 7 August 2019 Paper

Effects of irradiation modes in photodynamic therapy with azulene on singlet oxygen formation

Teerasak Damrongrungruang, Sutthichon Rattanayatikul, Nattapon Sontikarn, Boonsita Wuttiruk, Aroon Teerakapong, Patcharaporn Tippayawat

Proceedings Volume 11070, 110709L (2019) https://doi.org/10.1117/12.2525585

KEYWORDS: Oxygen, Photodynamic therapy, Luminescence, Light emitting diodes, Pulsed laser operation, Dentistry, Laser dentistry, Statistical analysis

Read Abstract +

Differences in the irradiation mode in Photodynamic Therapy result in different quantities of reactive oxygen species. We aimed to quantify the singlet oxygen formation in Photodynamic Therapy among various modes of red LED irradiation. Azulene powder was dissolved in ethanol/deionized water to obtain concentrations of 1, 10, and 100 μM. For all samples (90 μl/well), 10 μl of 10 mM dimethyl anthracene was added to each well of a 96-black well plate along with a singlet oxygen probe. After 30 minutes, irradiation was performed in arbitrary light units (638 nm, 0.5 watts, light bulb-to-well bottom distance = 2 mm) to obtain 4 or 40 J/cm² by any of the following irradiation modes: 1) a Continuous Mode, 2) a Fractional Mode (4 sessions, each session = 1/4 of final energy density resting 15 minutes between each session), or 3) a Pulse Mode (by alternative irradiation 300 msec with resting 700 msec). Samples were then subjected to singlet oxygen detection in a Varioskan (Thermo Fisher Scientific, USA) fluorescence microplate reader (excitation/emission wavelength = 375/436 nm). Measurements were performed in triplicate and the fluorescence intensity (relative singlet oxygen amount) was recorded. Kruskal Wallis with the Mann Whitney U test was performed, and p < 0.05 was determined as the significant difference. The amounts of relative singlet oxygen from PDT with higher energy density were relatively higher than those with lower energy. The continuous mode of irradiation resulted in a relatively higher singlet oxygen amount (p < 0.05). In conclusion, the continuous mode of red light irradiation with 40 J/cm² with azulene can produce the highest amount of singlet oxygen using a fluorescence probe.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years