Our current study using deuterium oxide probed stimulated Raman Scattering (DO-SRS) imaging provides new insights in brain lipid metabolism in female and male Drosophila individuals during aging processes. We found that the 3D volumetric distribution of newly synthesized lipids is heterogeneous, aged male and female flies have different metabolic activities in brain LDs, the glial LDs remain predominantly TAGs during aging, lipogenic diets improve brain functions by promoting lipid biosynthesis, and the IIS pathway mediates diets-regulated brain lipid metabolism.
We applied multiphoton fluorescence, SHG, and SRS to investigate In situ and chemical-specific identification of lipid and mitochondrial composition is crucial in analyzing the impacted subcellular components resulting in lipid accumulation, and differentiate mitochondrial associated bimolecular identification and related lipids’ spatial distribution in skeletal muscle cells between athletes and type 2 diabetes patients, to reveal differences in composition related to dysregulated metabolic pathways. Furthermore, quantitative SRS analysis revealed markers of irregular molecules for impacted mitochondrial function corresponding to decreased lipid metabolism seen in type 2 diabetes.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.