KEYWORDS: Acoustics, Signal processing, Tissue optics, Modulation, Imaging systems, Endoscopy, Intravascular ultrasound, Transducers, Absorption, Signal to noise ratio
Intravascular photoacoustics (IV-PA) is an emerging atherosclerosis imaging modality that provides chemical-specific optical information of arterial walls with acoustic depth penetration and resolution. As lipid composition of atherosclerotic plaques is considered to be one of the primary indicators for plaque vulnerability, many IV-PA applications are calibrated so as to target plaque necrotic cores. Based on the mode of optical excitation and the corresponding signal processing technique, IV-PA is categorized into two different modalities. The pulse-based IV-PA has been the universal IV-PA imaging mode with its high peak power and straightforward time-domain signal processing technique. As an alternative, the low power continuous-wave (CW)-based IV-PA has been under intense development as a radar-like frequency-domain signal processing modality. The two state-of-the-art types of IV-PA are reviewed in terms of their physics and imaging capabilities, with major emphasis on frequency-swept CW-based IV-PA that has been recently introduced in the field.
KEYWORDS: Photoacoustic spectroscopy, Signal processing, Radar, Signal to noise ratio, Intravascular ultrasound, Modulation, Tissues, Absorption, Interference (communication), Imaging systems
Lipid composition of atherosclerotic plaques is considered to be highly related to plaque vulnerability. Therefore, a specific diagnostic or imaging modality that can sensitively evaluate plaques’ necrotic core is desirable in atherosclerosis imaging. In this regard, intravascular photoacoustic (IVPA) imaging is an emerging plaque detection technique that provides lipid-specific chemical information from an arterial wall with great optical contrast and long acoustic penetration depth. While, in the near-infrared window, a 1210-nm optical source is usually chosen for IVPA applications since lipids exhibit a strong absorption peak at that wavelength, the sensitivity problem arises in the conventional single-ended systems as other arterial tissues also show some degree of absorption near that spectral region, thereby generating undesirably interfering photoacoustic (PA) signals. A theory of the high-frequency frequency-domain differential photoacoustic radar (DPAR) modality is introduced as a unique detection technique for accurate and molecularly specific evaluation of vulnerable plaques. By assuming two low-power continuous-wave optical sources at ∼1210 and ∼970 nm in a differential manner, DPAR theory and the corresponding simulation/experiment studies suggest an imaging modality that is only sensitive and specific to the spectroscopically defined imaging target, cholesterol.
Lipid composition of atherosclerotic plaques is considered to be one of the primary indicators of plaque vulnerability. Therefore, a specific diagnostic or imaging modality that can sensitively evaluate plaques’ necrotic core is highly desirable in atherosclerosis imaging. In this regard, intravascular photoacoustic (IVPA) imaging is an emerging plaque detection technique that provides lipid-specific chemical information from an arterial wall with great optical contrast and long acoustic penetration depth. Within the near-infrared window, a 1210-𝑛𝑚 optical source is usually chosen for IVPA applications as lipids exhibit a strong absorption peak at that wavelength due to the second overtone of the C-H bond vibration within the lipid molecules. However, other arterial tissues also show some degree of absorption near 1210 𝑛𝑚 and thus generate undesirably interfering PA signals. In this study, a theory of the novel Frequency-Domain Differential Photoacoustic Radar (DPAR) modality is introduced as an interference-free detection technique for accurate and reliable evaluation of vulnerable plaques. By assuming two low-power continuous-wave (CW) optical sources at ~ 1210 𝑛𝑚 and ~ 970 𝑛𝑚 in a differential manner, DPAR theory and the corresponding simulation study suggest a unique imaging modality that can efficiently suppress any undesirable absorptions and system noise, while dramatically improving PA sensitivity and specificity toward cholesterol contents of atherosclerotic plaques.
In this study, we present some examples of waveform engineering applications in frequency-domain photoacoustics (PA). An example of using linear frequency modulation (LFM) for PA spectroscopy is the capability of simultaneous probing/imaging with multiple wavelengths. Use of mismatched coded waveforms enables encoding the signal sources and, therefore, facilitates simultaneous probing and imaging. This method enables high frame rate functional imaging with reduced motion artifacts. Furthermore, it is shown than that phase of the PA cross-correlation induced with a LFM can yield the absolute absorption coefficient of the chromophores. This method is not affected by fluence attenuation or variation due to the absorption and scattering of the overlayer material.
This study explores wavelength-modulated differential photo-acoustic (WM-DPA) imaging for non-invasive early cancer detection via sensitive characterization of functional information such as hemoglobin oxygenation (sO2) levels. Well-known benchmarks of tumor formation such as angiogenesis and hypoxia can be addressed this way. While most conventional photo-acoustic imaging has almost entirely employed high-power pulsed lasers, frequency-domain photo-acoustic radar (FD-PAR) has seen significant development as an alternative technique. It employs a continuous wave laser source intensity-modulated and driven by frequency-swept waveforms. WM-DPA imaging utilizes chirp modulated laser beams at two distinct wavelengths for which absorption differences between oxy- and deoxygenated hemoglobin are minimum (isosbestic point, 805 nm) and maximum (680 nm) to simultaneously generate two signals detected using a standard commercial array transducer as well as a single-element transducer that scans the sample. Signal processing is performed using Lab View and Matlab software developed in-house.
Minute changes in total hemoglobin concentration (tHb) and oxygenation levels are detectable using this method since background absorption is suppressed due to the out-of-phase modulation of the laser sources while the difference between the two signals is amplified, thus allowing pre-malignant tumors to become identifiable. By regulating the signal amplitude ratio and phase shift the system can be tuned to applications like cancer screening, sO2 quantification and hypoxia monitoring in stroke patients. Experimental results presented demonstrate WM-DPA imaging of sheep blood phantoms in comparison to single-wavelength FD-PAR imaging. Future work includes the functional PA imaging of small animals in vivo.
This paper demonstrates the co-registration of ultrasound (US) and frequency domain photoacoustic radar (FD-PAR)
images with significant image improvement from applying image normalization, filtering and amplification techniques.
Achieving PA imaging functionality on a commercial Ultrasound instrument could accelerate clinical acceptance and
use. Experimental results presented demonstrate live animal testing and show enhancements in signal-to-noise ratio
(SNR), contrast and spatial resolution. The co-registered image produced from the US and phase PA images, provides
more information than both images independently.
Nanocrystalline TiO2 (nc-TiO2) in the anatase phase is widely used for photo-degrading organic pollutants in a variety of
environmental applications. Herein we show that slow photons in photonic crystals fashioned from nc-TiO2 can optically
amplify the photocatalytic efficiency as a result of the longer path length of light and increased probability in anatase
absorption, and that the optical amplification is tolerant to some degree of disorder. We investigated the photodegradation
of adsorbed methylene blue on inverse TiO2 opals (i-nc-TiO2-o) with different stop-band energies under
monochromatic and white light irradiation. By using template spheres with different diameters, the energy of the slow
photons was tuned in-and-out of the anatase electronic absorption, thereby allowing the systematic study of the effects of
photonic structure on the photo-degradation efficiency of TiO2. Under monochromatic irradiation at 370 nm, a
remarkable twofold enhancement was observed for i-nc-TiO2-o with stop-band at 345 nm, as a result of slow photon
coupling at 370 nm. Under white light (>300 nm) irradiation, an increase in the photo-degradation efficiency was
observed when the stop-band moves from 370 to 300 nm, as a result of slow photon coupling and the suppression of
stop-band reflection by the anatase absorption. By optimizing the energy of the photonic stop-band with respect to the
semiconductor electronic band gap, we effectively harvested slow photons in the dielectric part of the material to give
optically amplified photochemistry. Furthermore, we studied the effect of structural disorder on the photocatalytic
efficiency of the inverse opals by introducing different fractions and sizes of guest spheres into the opal template. We
found that half of the enhancement originally achieved by the inverse opal made from monodispersed spheres is
conserved when the domain size of the host spheres remains above a critical threshold. Such a high tolerance to
structural disorder provides strong support for the potential use of inverse TiO2 opals in environmental cleanup and
water treatment applications.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.