Anaplasma phagocytophilum is the intracellular bacteria that causes Human Granulocytic Anaplasmosis (HGA). While the skin is known as the entry site for the bacteria, HGA is characterized by systemic infection of A. phagocytophilum, but it is unknown how a localized infection of A. phagocytophilum leads to HGA. Here, we use a multimodal microscopy approach to image collagen and immune cell alterations due to A. phagocytophilum infection. Second-harmonic generation imaging revealed collagen fiber density decreased at the tick bite of infected skin. Additionally, significant differences in autofluorescence lifetime were observed due to A. phagocytophilum infection of human myeloid HL-60 cells.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.