The integration of a fluorescence microscopy/spectroscopy module in cell-based lab-on-a-chip systems is of high interest
for applications in cell-based diagnostics and substance evaluation in situ. We present an on-chip integrated lensless
fluorescence imaging module applying the principle of contact/proximate optical lithography. The pixel resolution is
comparable with a 4 x objective microscope. The module can be used for morphology and fluorescence imaging of
mammalian cells (15 - 20 μm) as well as for testing the concentration of a fluorescent substance. The biological samples
or solutions are sustained in disposable sterilized microfluidic chips with 1 μm thick silicon nitride (Si3N4) membranes.
These chips are assembled on the surface of a 5 megapixel colored CMOS image sensor array with 1.75 μm pixel size,
which is coated with an additional interference filter. Each culturing chip consists of a MEMS cavity chip and a PDMS
microfluidic interface. The surface of the CMOS image sensor is smoothened using SU-8 photoresist spin-coating for a
commercial grade interference filter (optical density ≥ 5) coating by Plasma-Ion Assisted Deposition thereafter. The
function is demonstrated by primary imaging results of the non-/fluorescent mammalian cells/microspheres as well as by
differentiating different concentrations of FITC solutions.
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