Accurate in situ monitoring of bacterial transport is important for increased understanding and improvement of bioremediation processes where microorganisms convert toxic compounds to more benign compounds. Bioremediation methods have become the preferred mechanism for the rehabilitation of hard to reach contaminated environments. In this study, we have used fluorescence spectroscopy to monitor the movement of fluorescently labelled bacteria (Rhodococcus erythropolis and Pseudomonas putida) within a bench-top column filled with a porous medium. In situ fluorescence measurements made using a fibre optic based instrument (‘optrode’) were compared to ex situ measurements made using a plate reader. In situ monitoring using this fibre optic based instrument is a promising alternative to ex situ measurements as the initial flow of bacteria is reliably observed. However, a greater understanding of the effect of the porous medium on fluorescence measurements is required to develop an accurate calibration for bacterial concentration based in situ measurements.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.