Mr. Yu-chia Tsao Profile

Yu-chia Tsao

at Forward Electronics Co Ltd

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Proceedings Article | 7 February 2008 Paper

Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila

Yu-Chia Tsao, Yi-Wen Yang, Woo-Hu Tsai, Tsong-Rong Yan

Proceedings Volume 6852, 685206 (2008) https://doi.org/10.1117/12.758498

KEYWORDS: Proteins, Gold, Fiber optics sensors, Surface plasmons, Sensors, Optical fibers, Biosensors, Halogens, Spectrum analysis, Multimode fibers

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Side-polished fiber immunosensor based on surface plasmon resonance (SPR) onto self-assembled protein A layer was proposed for the detection of Legionella pneumophila. A self-assembled protein A layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and activated by N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/ N-Hydroxysuccinimide (EDC/NHS). The formation of self-assembled protein A and gold layer on side-polished surface and the binding of antibody and antigen in series were confirmed by SPR response on spectrum. The binding protein A layer can improve the sensitivity, which indirectly supports the configurations that antibody layer is immobilized on the binding protein A layer with a well-ordered orientation. The surface morphology analyses of self-assembled protein A layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein A were demonstrated by SPR dip shifts on optical spectrum analyzer. The SPR fiber immunosensor for detection of L. pneumophila was developed and the detection limit was 10 CFU/ml with the SPR dip shift in wavelength from 1070 to 1105nm. The current fabrication technique of a SPR immunosensor using optical fiber for the detection of Legionella pneumophila could be applied to construct other biosensor.

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