We present the interferometric phase and absorption microscope (iPAM) platform for studying the biomechanics and fitness of sickle red blood cells (sRBCs). iPAM combines diffraction phase microscopy, tailored imaging media with customized optical properties, and extinction-based models to extract sRBC hemoglobin concentration and 3D structure from hundreds of cells in a single snapshot. Coupled with a customized microfluidic platform controlling cell deformation and oxygenation, we show that iPAM can provide a fitness index evaluating sRBC health that could act as an indicator detecting vaso-occlusive crisis risk and determine sickle cell disease treatment effectiveness.
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