Proceedings Article | 12 March 2024
Dennis Sourvanos, Timothy Zhu, Andreea Dimofte, Theresa Busch, Weibing Yang, Justin Burrell, Rodrigo Neiva, Todd Schoenbaum, Zhaoxu Chen, Kang Ko, Joseph Fiorellini
KEYWORDS: Tissues, Dosimetry, Medicine, Animal model studies, Anatomy, Statistical analysis, Muscles, In vivo imaging, Biological research, Optical properties
Red and near-infrared light therapies operate within a range of 600-1,100 nm. These wavelengths have been widely recognized with clinical versatility for stimulating, restoring, and regenerating damaged tissues. This therapy has found applications across various fields, including medicine, dentistry, dermatology, neurology, and ophthalmology. Accurately tailoring the calculation of light irradiation to specific tissue targets is fundamental for achieving optimal clinical efficacy. The precision of light delivery is often challenged by the varying optical properties of tissues, such as absorption, scattering, reflection, and refraction. To overcome this challenge, standardized treatment doses are necessary to ensure optimal light delivery and efficacy of the therapy.
The validation of the porcine model as a reliable and effective platform for in vitro and vivo dose-escalation trials is central to the success of this study. This model has been extensively studied and proven to be a valuable tool in biomedical research due to its anatomical and physiological similarities to humans. This model has the potential to refine irradiation parameters and investigate immunologic responses for consistent results. These parameters are crucial for attaining the desired therapeutic outcomes and vary depending on clinical conditions, treatment objectives, and the characteristics of the target tissue.
The porcine model has proven to be a highly versatile subject in a wide range of biological research fields. Its usefulness extends to studies on nerve regeneration, immunology, bone biology, and titanium osseointegration, among others. Researchers have found that the similarities between porcine and human physiology make this model an excellent tool for advancing our understanding of complex biological processes. The porcine model can facilitate various light dose escalation trial formats while enabling comprehensive assessments that integrate in vivo dosimetry. This model can also be expanded to characterize tissue optical properties, CT analysis, tissue histology, immune cell profiling, inflammatory response evaluation, histomorphometry, and biomechanical testing. This approach creates a translational framework to integrate in vivo dose-escalation trials and reinforces the importance of precision light dosimetry analysis.
