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Yet, experimental methods for determining the optical coefficients often yield different results. We describe several common methods of 2PA measurements and describe their features. As an example of the importance of applying the correct analysis to measurements, we fit experimental data and obtain values for multiphoton absorbers and accurately obtain their intrinsic values. Finally, we present the optical properties of several multiphoton materials used in biology.
We use a numerical method that simulates both time and space so that we can calculate on a near-instantaneous basis the absorption of laser light and electron populations. We can then calculate the intensity of the emitted signal and determine the overlap of the spectra.
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