Thrombosis remains a global health concern, necessitating research into its underlying mechanisms. Utilizing a high-speed bright-field microscope based on optical frequency-division multiplexing and microfluidics, we performed image-based single-cell profiling and temporal monitoring of circulating platelet aggregates that are the precursors to thrombosis. Our analysis encompassed 41 thrombosis patients, 110 COVID-19 patients, and 11 healthy individuals. By investigating the morphological changes of platelet aggregates under the influence of thrombosis, COVID-19, and COVID-19 vaccination, we observed distinct morphological alterations in platelet aggregates across different conditions, which shed light on the interplay between platelet aggregation and thrombotic events.
There is widespread concern about the safety of COVID-19 vaccinations related to platelet hyperactivity. However, their long-term influence on platelet activity remains unknown. We address this issue by applying a high-speed bright-field microscope based on optical frequency-division multiplexing and microfluidics for massive image-based analysis. We performed image-based single-cell profiling and temporal monitoring of circulating platelet aggregates in the blood samples of healthy human participants before and after they received three vaccination doses over a nearly one-year period. The results demonstrate no significant or persistent change in platelet activity after vaccine doses.
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