Dr. Saturnin S. Ombinda-Lemboumba Profile

Dr. Saturnin S. Ombinda-Lemboumba

Senior Researcher at Council for Scientific and Industrial Research

SPIE Involvement:

Author

Publications (49)

Proceedings Article | 27 March 2024 Presentation + Paper

Detection of viral pathogens using optical photonic techniques with the aid of selenium nanoparticles

Z. K. Mcotshana, L. Thwala, S. Ombinda-Lemboumba, R. Ramokolo, S. Thobakgale, M. Lugongolo, E. Van Steen, P. Mthunzi-Kufa

Proceedings Volume 12850, 1285008 (2024) https://doi.org/10.1117/12.3002381

KEYWORDS: Nanoparticles, Selenium, Antibodies, Localized surface plasmon resonances, Spectroscopy, Raman spectroscopy, Optical transmission, Glasses, Biosensors, YAG lasers

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Viral infections such as HIV and SARS-CoV-2 have significantly increased morbidity in humans and resulted in a significant number of fatalities globally, hence early detection is crucial, particularly at a point-of-care (POC) setting to prevent the spread of these diseases. Localized surface plasmon resonance (LSPR) and green light-based Transmission spectroscopy techniques were used in this study to assess real-time molecular interactions between virus-spiked and nonspiked samples. The current study focuses on integrating selenium nanoparticles (SeNPs) with different optical photonic techniques for enhanced detection of HIV. Selenium nanoparticles were synthesized and functionalized with antibodies specific to HIV. Before and after bioconjugation with viral secondary antibodies, the SeNPs were characterized using Ultraviolet–visible (UV-Vis) spectroscopy, Dynamic light scattering (DLS), High-resolution transmission electron microscopy (HRTEM), and Raman spectroscopy, to elucidate their properties and confirm the presence of functional groups. After that, the NPs were integrated with plasmonic systems and used for the enhanced detection of HIV in comparison to traditional LSPR and Transmission spectroscopy. Colloidal selenium nanoparticles were successfully synthesized, using ND: YAG laser. The orange-colored, spherically shaped nanoparticles were evenly distributed and easily resuspended. Anti-HIV antibodies conjugated to SeNPs were added after HIV-specific antibodies were successfully immobilized on a glass slide substrate to react with HIV pseudovirus. The pseudovirus was effectively identified by the use of Transmission Spectroscopy and LSPR techniques. The two optical techniques for HIV detection were more sensitive after integrating selenium nanoparticles, as compared to the conventional Transmission spectroscopy and LSPR methods. This improved and highly sensitive approach may be utilized to identify viral infections early, thus combating the spread of infectious diseases.

Proceedings Article | 13 March 2024 Presentation + Paper

Quantification of viral particles using a photonic crystal biosensor

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Charles Maphanga, Phumulani Michael Mcoyi, Ahmed El-Hussein, Patience Mthunzi-Kufa

Proceedings Volume 12860, 1286002 (2024) https://doi.org/10.1117/12.3002395

KEYWORDS: Photonic crystals, Antibodies, Biosensors, Spectroscopy, Biological samples, Biological research, Scanning electron microscopy, Nanoparticles, Gold nanoparticles

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Proceedings Article | 13 March 2024 Poster + Paper

The efficiency of surface plasmon resonance in measuring human immunodeficiency virus concentrations

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Lungile Nomcebo Thwala, Mabotse Tjale, Michael Phumulani Mcoyi, Patience Mthunzi-Kufa

Proceedings Volume 12860, 1286009 (2024) https://doi.org/10.1117/12.3002502

KEYWORDS: Localized surface plasmon resonances, Refractive index, Surface plasmons, Antibodies, Gold, Signal intensity, Gold nanoparticles, Biosensors, Statistical analysis, Metals

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Proceedings Article | 13 March 2024 Poster + Paper

Detection and quantification of iodine in biological fluids using photonic-based systems: UV-vis and transmission spectroscopy

Z. K. Mcotshana, L. Thwala, S. Ombinda-Lemboumba, R. Ramokolo, M. Lugongolo, E. Van Steen, P. Mthunzi-Kufa

Proceedings Volume 12822, 128220C (2024) https://doi.org/10.1117/12.3002412

KEYWORDS: Iodine, Spectroscopy, Selenium, Nanoparticles, UV-Vis spectroscopy, Absorbance, Biomedical optics, YAG lasers, Transmission electron microscopy, Neodymium

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Proceedings Article | 13 March 2024 Presentation + Paper

The effect of low-level laser therapy on severe acute respiratory syndrome coronavirus-2 infected cells

L. Mngwengwe, M. Lugongolo, S. Ombinda-Lemboumba, Y. Ismail, P. Mthunzi-Kufa

Proceedings Volume 12826, 1282606 (2024) https://doi.org/10.1117/12.3002453

KEYWORDS: Laser irradiation, Fluorescence, Laser therapeutics, Dyes, Cell death, Oxygen, In vitro testing, Error control coding, Absorbance, Tissues

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Proceedings Article | 13 March 2024 Poster + Paper

HEK293/ACE2 cells' response to severe acute respiratory syndrome coronavirus-2 infection and low-level-laser therapy under microscopy

L. Mngwengwe, M. Lugongolo, S. Ombinda-Lemboumba, Yaseera Ismail, P. Mthunzi-Kufa

Proceedings Volume 12826, 1282609 (2024) https://doi.org/10.1117/12.3002406

KEYWORDS: Scanning electron microscopy, Laser irradiation, Transmission electron microscopy, Electron microscopes, Tissues, Microscopy

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Proceedings Article | 12 March 2024 Presentation + Paper

The use of gold nanoparticles as a signal enhancer in LSPR for the detection of SARS-CoV-2

M. H. Tlomatsane, L. Thwala, Z. K. Mcotshana, S. Ombinda-Lemboumba, C. Williamson, P. Mthunzi-Kufa

Proceedings Volume 12840, 1284005 (2024) https://doi.org/10.1117/12.3002379

KEYWORDS: Gold nanoparticles, Localized surface plasmon resonances, Antibodies, Biosensors, COVID 19, Signal detection, Transmission electron microscopy, Sodium, Viruses, Metals

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Proceedings Article | 12 March 2024 Presentation + Paper

Detection of mutations using a localized surface plasmon resonance biosensor

M. P. Mcoyi, M. Lugongolo, C. Williamson, K. Mpofu, S. Ombinda-Lemboumba, P. Mthunzi-Kufa

Proceedings Volume 12840, 128400A (2024) https://doi.org/10.1117/12.3002281

KEYWORDS: Localized surface plasmon resonances, Signal intensity, Biosensors, Gold nanoparticles, Molecular interactions, Glasses, Surface plasmons, Signal detection, Sensors, Refractive index

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Proceedings Article | 12 March 2024 Poster + Paper

Biosensing multidrug-resistant TB genes using SPR

S. Chauke, S. Ombinda-Lemboumba, F. Dube, P. Mthunzi-Kufa

Proceedings Volume 12840, 128400H (2024) https://doi.org/10.1117/12.3002305

KEYWORDS: Sensors, Biosensors, Biosensing, Reflection, Particles, Glasses, Resistance, Refractive index, Gold

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Proceedings Article | 12 March 2024 Presentation + Paper

Optical biosensing of multidrug-resistant tuberculosis (TB) genes

S. Chauke, S. Ombinda-Lemboumba, F. Dube, P. Mthunzi-Kufa

Proceedings Volume 12840, 128400B (2024) https://doi.org/10.1117/12.3002279

KEYWORDS: Signal intensity, Glasses, Ocean optics, Sensors, Target detection, Biosensors, Biosensing, Biological samples, Statistical analysis, Signal detection

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Tuberculosis (TB) remains one of the most important infectious diseases globally, killing approximately 1.5 million people annually. The burden of infection is disproportionately high in low-income and resource-limited settings. This disparity is exacerbated by the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis (Mtb), the bacterium that causes TB. Early detection and treatment of TB remain key strategies to reduce the spread and disease progression to drug-resistant forms of TB. However, this is hampered by slow, insensitive diagnostic methods, particularly for the detection of drug-resistant forms and in patients with human immunodeficiency virus infection (HIV). There are currently several rapid TB diagnostics, but most are limited due to costs and accessibility. Several genes, such as catalase-peroxidase (katG) and enoyl reductase (inhA) genes, contain mutations that are responsible for drug resistance. One of the initial objectives of this study was to use an optical-based system to detect katG and inhA genes. Deoxyribonucleic acid (DNA) probes, specific for katG and inhA, were used as biorecognition elements to capture katG and inhA target DNA. The katG and inhA gene-specific DNA probes were immobilized on a coated glass substrate before the target DNA was introduced for detection. As a negative control, a mismatched probe, unspecific to both genes was used for confirmation of the absence of the two genes in the experimental setup. The optical setup was used for the analysis of the binding interactions occurring on the coated glass substrate. The specificity and sensitivity of the coated glass substrate successfully detected the binding interactions through the changes in the transmitted intensity. The transmitted intensity further indicated the kinetics associated with DNA hybridization occurring between the target DNA and the capture probe. This is the initial step to potentially detecting drug-resistant mutations using optical-based biosensors at a point-of-care setting.

Proceedings Article | 12 March 2024 Presentation + Paper

Development and evaluation of surface plasmon resonance phase difference for tuberculosis detection

Sinegugu Nzuza, Saturnin Ombinda-Lemboumba, Heidi Abrahamse, Patience Mthunzi-Kufa

Proceedings Volume 12840, 1284009 (2024) https://doi.org/10.1117/12.3002334

KEYWORDS: Surface plasmons, Biosensors, Antibodies, Biological samples, Biosensing, Biological research, UV-Vis spectroscopy, Statistical analysis, Polarization, Bacteria

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Proceedings Article | 12 March 2024 Poster + Paper

Chitosan/Au nanocomposites-based LSPR sensor for SARS-CoV-2 detection

M. H. Tlomatsane, L. Thwala, Z. K. Mcotshana, S. Ombinda-Lembouba, C. Williamson, P. Mthunzi-Kufa

Proceedings Volume 12840, 128400L (2024) https://doi.org/10.1117/12.3002495

KEYWORDS: Gold nanoparticles, Localized surface plasmon resonances, Transmission electron microscopy, Antibodies, Sensors, Solubility, COVID 19, Target detection, Proteins, Nanocomposites

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Proceedings Article | 12 March 2024 Poster + Paper

Evaluation of the limit of detection of the localized surface plasmon resonance label-free biosensor

M. P. Mcoyi, M. Lugongolo, C. Williamson, K. Mpofu, S. Ombinda-Lemboumba, S. Ndlovu, P. Mthunzi-Kufa

Proceedings Volume 12840, 128400G (2024) https://doi.org/10.1117/12.3002239

KEYWORDS: Localized surface plasmon resonances, Biosensors, Biosensing, Signal intensity, Molecules, Calibration, Titanium, Gold nanoparticles, Surface plasmons, Sensors

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Proceedings Article | 12 March 2024 Poster + Paper

Improved sensitivity of tuberculosis antigen MPT64 detection using SPR phase difference

Sinegugu Nzuza, Saturnin Ombinda-Lemboumba, Heidi Abrahamse, Patience Mthunzi-Kufa

Proceedings Volume 12840, 128400J (2024) https://doi.org/10.1117/12.3002359

KEYWORDS: Antibodies, Phase shifts, Biological research, Surface plasmons, Biosensing, Reflection, Biosensors, Biomolecules, Statistical analysis, Molecular interactions

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Proceedings Article | 17 March 2023 Paper

Optical biosensing of human immunodeficiency virus on a gold coated surface

Masixole Lugongolo, Saturnin Ombinda-Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 12396, 123960B (2023) https://doi.org/10.1117/12.2647722

KEYWORDS: Antibodies, Optical biosensors, Gold nanoparticles, Gold, Signal intensity, Transmittance, Optical surfaces, Biosensing, Biological research, Surface plasmons

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Proceedings Article | 16 March 2023 Presentation + Paper

Optical biosensing of mycolic acid biomarker for TB diagnosis

Charles Maphanga, Sello Manoto, Saturnin Ombinda-Lemboumba, Yaseera Ismail, Patience Mthunzi-Kufa

Proceedings Volume 12387, 123870E (2023) https://doi.org/10.1117/12.2650013

KEYWORDS: Antibodies, Biosensing, Gold, Biosensors, Scanning electron microscopy, Biological research, Spectroscopy, Signal detection, Thin films, Statistical analysis

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Proceedings Article | 16 March 2023 Poster + Paper

Photonic crystal-based biosensor chip development for TB diagnosis

Charles Maphanga, Saturnin Ombinda-Lemboumba, Yaseera Ismail, Patience Mthunzi-Kufa

Proceedings Volume 12387, 123870K (2023) https://doi.org/10.1117/12.2650057

KEYWORDS: Biosensing, Antibodies, Biosensors, Photonic crystals, Signal detection, Refractive index, Biological research, Atomic force microscopy, Biomolecules, Statistical analysis

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Proceedings Article | 7 March 2023 Presentation + Paper

Detection of human immunodeficiency virus on a photonic crystal-based platform

Masixole Lugongolo, Saturnin Ombinda-Lemboumba, Charles Maphanga, Patience Mthunzi-Kufa

Proceedings Volume 12377, 1237702 (2023) https://doi.org/10.1117/12.2647726

KEYWORDS: Photonic crystals, Antibodies, Gold nanoparticles, Biosensors, UV-Vis spectroscopy, Scanning electron microscopy, Point-of-care devices, Diagnostics, Crystals, Spectroscopy

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Proceedings Article | 7 March 2023 Poster + Paper

Low-level laser therapy for treatment of severe acute respiratory syndrome 2 infection

L. Mngwengwe, M. Lugongolo, S. Ombinda-Lemboumba, P. Mthunzi-Kufa

Proceedings Volume 12377, 123770H (2023) https://doi.org/10.1117/12.2648850

KEYWORDS: Laser irradiation, Laser therapeutics, Tissues, Luminescence, Pulmonary disorders, Near infrared, Biological samples, Semiconductor lasers, Continuous wave operation, Beam diameter

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Proceedings Article | 1 August 2021 Presentation + Paper

Examining HIV infected cells using optical trapping and Raman spectroscopy

Masixole Lugongolo, Saturnin Ombinda-Lemboumba, Sello Manoto, Patience Mthunzi-Kufa

Proceedings Volume 11798, 117982A (2021) https://doi.org/10.1117/12.2595766

KEYWORDS: Raman spectroscopy, Optical tweezers, Spectroscopy, Molecules, Chemical analysis, Luminescence, Semiconductor lasers, Molecular spectroscopy, Light scattering, Viruses

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Proceedings Article | 5 March 2021 Poster + Presentation + Paper

Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies

Charles Maphanga, Sello Manoto, Saturnin Ombinda-Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 11651, 116510X (2021) https://doi.org/10.1117/12.2578789

KEYWORDS: Nanoparticles, Gold, Transmission electron microscopy, Diagnostics, Adsorption, Surface plasmons, Dielectrics, Biosensors, Biosensing, Biological research

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Proceedings Article | 5 March 2021 Presentation + Paper

Surface plasmon resonance (SPR) based biosensor for mycobacterium tuberculosis diagnosis

Charles Maphanga, Saturnin Ombinda-Lemboumba, Sello Manoto, Patience Mthunzi-Kufa

Proceedings Volume 11651, 1165103 (2021) https://doi.org/10.1117/12.2578728

KEYWORDS: Surface plasmons, Biosensors, Signal detection, Gold, Biosensing, Scanning electron microscopy, Diagnostics, Biological research, UV-Vis spectroscopy, Proteins

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Proceedings Article | 5 March 2021 Presentation + Paper

Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor

M. Lugongolo, S. Manoto, C. Maphanga, S. Ombinda-Lemboumba, S. Thobakgale, P. Mthunzi-Kufa

Proceedings Volume 11661, 116610M (2021) https://doi.org/10.1117/12.2578317

KEYWORDS: Surface plasmons, Biosensors, Sensors, Resistance, Refractive index, Polymers, Optical testing, Molecular interactions

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Proceedings Article | 2 March 2020 Presentation + Paper

Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy

Lebogang Thobakgale, Sello Manoto, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 11269, 1126905 (2020) https://doi.org/10.1117/12.2546479

KEYWORDS: Raman spectroscopy, Surface enhanced Raman spectroscopy, Thin films, Biosensors, Gold, Calibration

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Proceedings Article | 21 February 2020 Presentation + Paper

Smartphone biosensing for point of care diagnostics

Sello Lebohang Manoto, Chemist Mabena, Rudzani Malabi, Saturnin Ombinda-Lemboumba, Ahmed El-Hussein, Mohammed Kasem, Masixole Lugongolo, Patience Mthunzi-Kufa

Proceedings Volume 11258, 112580J (2020) https://doi.org/10.1117/12.2546444

KEYWORDS: Rhodamine, Point-of-care devices, Diagnostics, Biosensing, Biosensors, Light emitting diodes, Cameras, Calibration, Spectroscopy, Internet

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Proceedings Article | 21 February 2020 Presentation + Paper

Development of collinear transmission plasmonic biosensor for detection of HIV-1

Saturnin Ombinda-Lemboumba, Sello Manoto, Charles Maphanga, Rudzani Malabi, Patience Mthunzi-Kufa

Proceedings Volume 11257, 112570L (2020) https://doi.org/10.1117/12.2546496

KEYWORDS: Glasses, Gold, Sensors, Nanoparticles, Transmittance, Plasmonics, Biosensors, Ocean optics, CCD cameras, Point-of-care devices

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Proceedings Article | 21 February 2020 Paper

Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing

Sello Manoto, Ahmed El-Hussein, Rudzani Malabi, Lebogang Thobakgale, Saturnin Ombinda-Lemboumba, Yasser Attia, Mohammed Kasem, Patience Mthunzi-Kufa

Proceedings Volume 11258, 112580O (2020) https://doi.org/10.1117/12.2546497

KEYWORDS: Glasses, Raman spectroscopy, Biosensing, Nanoparticles, Atomic force microscopy, Biosensors, Water, Physics, Chemistry, Proteins

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Proceedings Article | 20 February 2020 Paper

Raman spectroscopy and gold thin film for biosensing and detection

Saturnin Ombinda-Lemboumba, Sello Manoto, Charles Maphanga, Rudzani Malabi, Lebogang Thobakgale, Masixole Lugongolo, Patience Mthunzi-Kufa

Proceedings Volume 11251, 112512P (2020) https://doi.org/10.1117/12.2546617

KEYWORDS: Raman spectroscopy, Gold, Nanoparticles, Glasses, Raman scattering, Sensors, Surface enhanced Raman spectroscopy, Molecules, Thin films, Biosensing

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Proceedings Article | 20 February 2020 Paper

Characterisation of a Bessel beam optical cell sorting system using microspheres

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Sello Manoto, Patience Mthunzi-Kufa

Proceedings Volume 11238, 1123804 (2020) https://doi.org/10.1117/12.2541971

KEYWORDS: Bessel beams, Refractive index, Particles, Optical sorting, Silica, Mirrors, Beam propagation method, Microscopes, Objectives, Axicons

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Proceedings Article | 20 February 2020 Presentation + Paper

Optical biosensing of mycobacterium tuberculosis for point-of-care diagnosis

Charles Maphanga, Sello Manoto, Saturnin Ombinda-Lemboumba, Lerato Hlekelele, Patience Mthunzi-Kufa

Proceedings Volume 11251, 112510R (2020) https://doi.org/10.1117/12.2545842

KEYWORDS: Biosensors, Biosensing, Scanning electron microscopy, Point-of-care devices, Biological research, Gold, Glasses, Refractive index, Spectrographs

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Proceedings Article | 13 February 2020 Presentation + Paper

Surface enhanced raman spectroscopy on polymer-graphene oxide scaffolds for drug screening applications

Lebogang Thobakgale, Sello Manoto, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 11246, 112460X (2020) https://doi.org/10.1117/12.2546455

KEYWORDS: Graphene, Raman spectroscopy, Oxides, Glasses, Surface enhanced Raman spectroscopy, Polymers, Nanomaterials, Coating, Molecules, Molecular self-assembly

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Proceedings Article | 7 March 2019 Paper

Biocongugation of gold nanoparticles for surface plasmon resonance sensor

Sello Manoto, Rudzani Malabi, Saturnin Ombinda-Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 10894, 108941D (2019) https://doi.org/10.1117/12.2509952

KEYWORDS: Sensors, Gold, Refractive index, Nanoparticles, Surface plasmons, Metals, Scanning electron microscopy, Prisms, Electrons, Biosensing

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Proceedings Article | 7 March 2019 Paper

Design and FDTD simulation of photonic crystal based sensor for biosensing applications

Sello Manoto, Chemist Mabena, Rudzani Malabi, Saturnin Ombinda-Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 10895, 1089510 (2019) https://doi.org/10.1117/12.2509855

KEYWORDS: Finite-difference time-domain method, Refractive index, Photonic crystals, Biosensing, Sensors, Electromagnetism, Waveguides, Biological research, Monte Carlo methods, Optical design

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Proceedings Article | 7 March 2019 Paper

Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool

Rudzani Malabi, Sello Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10894, 108940P (2019) https://doi.org/10.1117/12.2509850

KEYWORDS: Gold, Biological research, Nanoparticles, Surface plasmons, Scanning electron microscopy, Point-of-care devices, Biosensing, Diagnostics, Sensors, Chemical analysis

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Proceedings Article | 7 March 2019 Paper

Growth and characterisation of gold thin film layer using an ebeam evaporation system for surface plasmon resonance applications

Rudzani Malabi, Sello Manoto, Saturnin Ombinda-Lemboumba, Sfundo Khanyile , Saleh Khamlich , Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10894, 108941E (2019) https://doi.org/10.1117/12.2509972

KEYWORDS: Gold, Thin films, Surface plasmons, Thin film coatings, Coating, Metals, Scanning electron microscopy, X-ray diffraction, Glasses, Optical properties

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Proceedings Article | 1 March 2019 Presentation + Paper

Near infrared femtosecond laser-induced bacterial inactivation

Charles Maphanga, Sello Manoto, Saturnin Ombinda-Lemboumba, Olayinka Osualale, Patience Mthunzi-Kufa

Proceedings Volume 10876, 108760K (2019) https://doi.org/10.1117/12.2509806

KEYWORDS: Microorganisms, Acquisition tracking and pointing, Bacteria, Near infrared, Femtosecond phenomena, Laser irradiation, Ultraviolet radiation, Microscopes, Pulsed laser operation, Viruses

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Proceedings Article | 22 February 2019 Paper

Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres

Lebogang Thobakgale, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa, Rudzani Malabi

Proceedings Volume 10884, 1088416 (2019) https://doi.org/10.1117/12.2509944

KEYWORDS: Raman spectroscopy, Light scattering, Molecules, Data processing, Raman scattering, Polymers, Scattering, CCD cameras, Statistical analysis, Polarizability

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Proceedings Article | 20 February 2019 Presentation + Paper

Investigation and calibration of non-essential amino acids using a custom built Raman spectroscopy system

Lebogang Thobakgale, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 10885, 108850N (2019) https://doi.org/10.1117/12.2509839

KEYWORDS: Raman spectroscopy, Proteins, Calibration, Luminescence, Signal detection, Nanomaterials, Remote sensing, Standards development, Raman scattering, Point-of-care devices

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Proceedings Article | 20 February 2018 Paper

Label-free detection of HIV-1 infected cells via integration of optical tweezers and photoluminescence spectroscopy

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Luyanda Lunga Noto, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10501, 105011G (2018) https://doi.org/10.1117/12.2290168

KEYWORDS: Luminescence, Spectroscopy, Optical tweezers, Diagnostics, Biological research, In vitro testing

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Proceedings Article | 13 February 2018 Presentation + Paper

Femtosecond laser assisted photo-transfection and differentiation of mouse embryonic stem cells

Lebogang Thobakgale, Sello Manoto, Satuurnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10492, 1049205 (2018) https://doi.org/10.1117/12.2290206

KEYWORDS: Stem cells, Luminescence, Acquisition tracking and pointing, Femtosecond phenomena, Green fluorescent protein, Microscopes, Pulsed laser operation, Statistical analysis, Organisms, Genetics

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Proceedings Article | 22 February 2017 Paper

Could low level laser therapy and highly active antiretroviral therapy lead to complete eradication of HIV-1 in vitro?

Masixole Yvonne Lugongolo, Sello Lebohang Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10075, 100750B (2017) https://doi.org/10.1117/12.2252210

KEYWORDS: Laser therapeutics, Laser irradiation, In vitro testing, Injuries, Near infrared, Cell death, Tissues, Semiconductor lasers, Humidity, Continuous wave operation

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Proceedings Article | 22 February 2017 Paper

Investigation of HIV-1 infected and uninfected cells using the optical trapping technique

S. Ombinda-Lemboumba, R. Malabi, M. Y. Lugongolo, S. Thobakgale, S. Manoto, P. Mthunzi-Kufa

Proceedings Volume 10075, 100750M (2017) https://doi.org/10.1117/12.2252294

KEYWORDS: Optical tweezers, Objectives, Spectroscopy, Transmittance, Optical spectroscopy, Chemical analysis, Laser spectroscopy, Absorption, Biological research, Molecular spectroscopy

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Proceedings Article | 16 February 2017 Paper

Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: differentiation studies

Lebogang Thobakgale, Sello Lebohang Manoto, Satuurnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10068, 100681U (2017) https://doi.org/10.1117/12.2252255

KEYWORDS: Stem cells, Tissue engineering, Molecules, Biological research, Luminescence, Acquisition tracking and pointing, Femtosecond phenomena, Laser induced fluorescence, Genetics, Tissues, Absorbance, Receptors

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Proceedings Article | 15 February 2017 Presentation + Paper

In-vitro photo-translocation of antiretroviral drug delivery into TZMbl cells

Rudzani Malabi, Sello Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10062, 1006204 (2017) https://doi.org/10.1117/12.2252238

KEYWORDS: In vitro testing, Therapeutics, Plasma, Acquisition tracking and pointing, Luminescence, Transient nonlinear optics, Beam splitters, Objectives

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Proceedings Article | 15 February 2017 Presentation + Paper

Phototransfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Lebogang Thobakgale, Sello Lebohang Manoto, Saturnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10062, 1006206 (2017) https://doi.org/10.1117/12.2252188

KEYWORDS: Femtosecond phenomena, Stem cells, Genetics, Tissues, Molecules, Tissue engineering, Laser irradiation, Laser ablation, Optical properties, Luminescence, Acquisition tracking and pointing, Absorbance, Pulsed laser operation, Proteins, Objectives, Chemical analysis

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Proceedings Article | 15 February 2017 Presentation + Paper

Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: in-vitro studies

Charles Maphanga, Saturnin Ombinda-Lemboumba, Sello Manoto, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10062, 1006205 (2017) https://doi.org/10.1117/12.2252291

KEYWORDS: In vitro testing, Femtosecond phenomena, Gaussian beams, Statistical analysis, Cancer, In vivo imaging, Titanium, Sapphire lasers, Beam controllers, Control systems, Virtual colonoscopy, Objectives, Mirrors, Laser therapeutics

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Proceedings Article | 15 February 2017 Paper

Pros and cons of characterising an optical translocation setup

Charles Maphanga, Rudzani Malabi, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10062, 100621A (2017) https://doi.org/10.1117/12.2252426

KEYWORDS: Plasma, Femtosecond phenomena, Ultrafast phenomena, Electrons, Pulsed laser operation, Ovary, Genetics, Titanium, Sapphire, Optical amplifiers, Objectives, Microscopes, Camera shutters, Mirrors

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Proceedings Article | 8 February 2017 Paper

Phototodynamic activity of zinc monocarboxyphenoxy phthalocyane (ZnMCPPc) conjugated to gold silver (AuAg) nanoparticles in melanoma cancer cells

Sello Manoto, David Oluwole, Rudzani Malabi, Charles Maphanga, Saturnin Ombinda-Lemboumba, Tebello Nyokong, Patience Mthunzi-Kufa

Proceedings Volume 10047, 1004716 (2017) https://doi.org/10.1117/12.2252838

KEYWORDS: Nanoparticles, Cancer, Photodynamic therapy, Melanoma, Gold, Silver, Cell death, Picosecond phenomena

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Proceedings Article | 8 February 2017 Presentation + Paper

Phototoxic effects of free phthalocyanine and phthalocyanine conjugated to gold nanoparticles for targeted photodynamic therapy of melanoma cancer

Sello Manoto, David Oluwole, Rudzani Malabi, Charles Maphanga, Saturnin Ombinda-Lemboumba, Tebello Nyokong, Patience Mthunzi-Kufa

Proceedings Volume 10047, 100470D (2017) https://doi.org/10.1117/12.2252682

KEYWORDS: Photodynamic therapy, Gold, Nanoparticles, Melanoma, Cancer, Oxygen, Cell death, Acquisition tracking and pointing

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