The results of applying algorithms for reconstructing parameters characterizing the linear dichroism of networks of biological crystals in differentiating changes in optical anisotropy associated with varying degrees of severity of pathology, pre-cancerous (atrophy and endometrial polyp) states of the cervix are presented.
The results of a statistical analysis of the distributions of characteristic values of the Stokes vector fourth parameter (crystallization parameter - CP) of the digital vector-parametric microscopic image of healthy spleen and with spleen with sepsis histological sections are presented. We determined the statistical significance of differentiation of vector-parametric maps of the microscopic image of histological sections of rat spleens from control group 1 and research groups 2-4 with different severity of septic pathology. The most diagnostic-sensitive statistical criteria for differentiating vector-parametric maps of microscopic images of histological sections of the spleen are established.
A new digital technique of objective differential diagnosis of the septic process severity was developed and tested experimentally by polarization-phase mapping of microscopic images of histological sections of the liver of laboratory rats based on statistical and information analysis of phase shift distributions.
For differential diagnosis of severity of the septic process, the method of mapping vector-parametric microscopic images of the polycrystalline component of histological sections of the internal organs of rats was used. A statistical analysis of the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats was carried out. The relationships between the values of statistical moments of the 1st - 2nd orders of magnitude characterizing the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats and the severity of the septic process are determined. Quantitative parameters that are most sensitive to septic conditions have been established, which ensure the implementation of statistically confidance differentiation of histological sections of the internal organs of laboratory rats. From the standpoint of evidence-based medicine, the operational characteristics of the diagnostic forces of the vector-parametric microscopy method are established with an estimate of the number of characteristic values of the fourth Stokes vector of polarization images of histological sections of the internal organs of laboratory rats.
In this paper are presented: structural-logical diagram and design of a phasemetric study of microscopic images of blood films of laboratory rats; model analysis of the polycrystalline structure of blood films of laboratory rats; structurally logical diagram and characteristics of the optical arrangement of the system of phasemetric mapping of microscopic images of blood films of laboratory rats; algorithms for statistical processing of experimental data of a system for phasemetric mapping of microscopic images of blood films of laboratory rats; information analysis algorithms to determine the strength of the diagnostic method of phasemetric mapping of microscopic images of blood films of laboratory rats by establishing a set of operational characteristics - sensitivity, specificity and accuracy.
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