Label-free and nondestructive mid-infrared (MIR) vibrational hyperspectral imaging has emerged as a valuable ex-vivo tool for biomedical tissue analysis. However, due to the chemically complex and heterogeneous composition of tissue specimens, the analytical performance of conventional MIR spectral histopathology is limited. We introduce an innovative MIR spectrochemical tissue imaging modality that uses plasmonic metasurfaces to support strong surface-localized electromagnetic fields, enabling the capture of quantitative molecular maps of large-area brain tissue sections. Our surface-enhanced chemical imaging method has broad potential applications in both translational biomedical research and diagnostic clinical histopathology.
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